Enriched preparation of human fetal multipotential neural stem cells

Abstract

The present invention relates to a method of separating multipotential neural progenitor cells from a mixed population of cell types. This method includes selecting a promoter which functions selectively in the neural progenitor cells, introducing a nucleic acid molecule encoding a fluorescent protein under control of said promoter into all cell types of the mixed population of cell types, allowing only the neural progenitor cells, but not other cell types, within the mixed population to express said fluorescent protein, identifying cells of the mixed population of cell types that are fluorescent, which are restricted to the neural progenitor cells, and separating the fluorescent cells from the mixed population of cell types, wherein the separated cells are restricted to the neural progenitor cells. The present invention also relates to an isolated human musashi promoter and an enriched or purified preparation of isolated multipotential neural progenitor cells.

1 . An isolated human musashi promoter. 
     
     
         2 . An isolated human musashi promoter according to  claim 1 , wherein the musashi promoter has a nucleotide sequence of SEQ ID NO:1. 
     
     
         3 . An isolated human nestin enhancer. 
     
     
         4 . An isolated human nestin enhancer according to  claim 3 , wherein the nestin enhancer has a nucleotide sequence of SEQ ID NO:2.