US2009130700A1PendingUtilityA1

Device and Method for Determining the Concentration of a Substance

54
Assignee: INCE CANPriority: Jul 6, 2005Filed: Jul 6, 2006Published: May 21, 2009
Est. expiryJul 6, 2025(expired)· nominal 20-yr term from priority
Inventors:Can InceEgbert G. Mik
A61K 49/0021A61K 49/0036A61K 49/0056G01N 21/6408Y10T436/207497
54
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

The invention provides a method for determining a concentration of a substance in a compartment comprising exciting an endogenous compound, or a functional part, derivative, analogue or precursor thereof, measuring the lifetime of the luminescence and/or transient absorption exhibited by said compound, functional part, derivative, analogue and/or precursor, and correlating said lifetime with the concentration of said substance.

Claims

exact text as granted — not AI-modified
1 . A method for determining a concentration of a substance in a compartment, the method comprising:
 exciting an endogenous compound or a functional part, derivative, analogue and/or precursor of said compound, wherein said endogenous compound or functional part, derivative, analogue and/or precursor, if excited, exhibits a luminescence and/or transient absorption of which the lifetime is dependent on said substance,   measuring the lifetime of the luminescence and/or transient absorption exhibited by said compound, functional part, derivative, analogue and/or precursor, and   correlating said lifetime with said concentration.   
   
   
       2 . The method according to  claim 1 , wherein said substance comprises oxygen. 
   
   
       3 . The method according to  claim 1 , wherein said luminescence and/or transient absorption comprises delayed fluorescence and/or triplet-triplet absorption. 
   
   
       4 . The method according to  claim 1 , wherein said compound comprises a compound capable of being excited to a triplet state. 
   
   
       5 . The method according to  claim 1 , wherein said compound comprises a porphyrin. 
   
   
       6 . The method according to  claim 1 , wherein said compound comprises a protoporphyrin. 
   
   
       7 . The method according to  claim 5 , wherein said compound comprises protoporphyrin IX. 
   
   
       8 . The method according to  claim 1 , wherein said compound is photo-excited. 
   
   
       9 . The method according to  claim 1 , wherein said compartment comprises a cell. 
   
   
       10 . The method according to  claim 1 , wherein said compartment comprises an organelle. 
   
   
       11 . The method according to  claim 10 , wherein said organelle comprises a mitochondrion. 
   
   
       12 . The method according to  claim 1 , wherein said compartment comprises at least part of a tissue. 
   
   
       13 . The method according to  claim 1 , wherein said compartment comprises an organ. 
   
   
       14 . The method according to  claim 1 , wherein said compartment comprises a tumor. 
   
   
       15 . The method according to  claim 1 , wherein said compartment comprises the microcirculation. 
   
   
       16 . The method according to  claim 12 , wherein said tissue is present in a culture medium. 
   
   
       17 . The method according to  claim 1 , wherein said compartment comprises a cell suspension. 
   
   
       18 . The method according to  claim 1 , wherein said lifetime is compared with a reference. 
   
   
       19 . The method according to  claim 1 , wherein said lifetime is measured within four hours. 
   
   
       20 . The method according to  claim 1 , wherein said lifetime is measured in the time-domain. 
   
   
       21 . The method according to  claim 1 , wherein said lifetime is measured in the frequency-domain. 
   
   
       22 . The method according to  claim 1 , wherein multi-photon excitation is applied. 
   
   
       23 . The method according to  claim 1 , wherein two-photon excitation is applied. 
   
   
       24 . A device for determining a concentration of a substance in a compartment, the device comprising:
 means for exciting an endogenous compound or a functional part, derivative, analogue and/or precursor thereof, wherein said endogenous compound, part, derivative, analogue and/or precursor, if excited, exhibits a luminescence and/or transient absorption of which the lifetime is dependent on said substance, and   means for measuring said lifetime.   
   
   
       25 . The device according to  claim 24 , comprising a combination of a prism and a bandpass filter. 
   
   
       26 . The device according to  claim 24 , comprising a fast shutter in front of a PMT. 
   
   
       27 . The device of  claim 24 , comprising a semi-conductor device. 
   
   
       28 . The device of  claim 24 , comprising an imaging device capable of oxygen mapping. 
   
   
       29 .- 31 . (canceled)

Join the waitlist — get patent alerts

Track US2009130700A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.