Solid support

Abstract

The present invention provides a solid support for performing steps of isolation of cell or extraction and purification of nucleic acid, safely, easily, efficiently, and with high yield in the genetic test for investigating the presence of pathogenic bacterial infection. A solid support for binding with cell as an embodiment of the above-described solid support, comprises a polypeptide having capability of binding with mycolic acid-containing glycolipid which is immobilized on the surface of a carrier. In addition, a solid support for binding with nucleic acid as another embodiment of the above-described solid support, comprises a polypeptide having capability of binding with nucleic acid which is immobilized on the surface of a carrier.

1 . A solid support, comprising a polypeptide having a capability of binding with mycolic acid-containing glycolipid which is immobilized on the surface of a carrier. 
   
   
       2 . The solid support according to  claim 1 , wherein the carrier is a magnetic carrier. 
   
   
       3 . A solid support, comprising a polypeptide having a capability of binding with nucleic acid which is immobilized on the surface of a carrier. 
   
   
       4 . The solid support according to  claim 3 , wherein the carrier is a magnetic carrier. 
   
   
       5 . The solid support according to  claim 1 , wherein the polypeptide has a capability of binding with mycolic acid-containing glycolipid and nucleic acid. 
   
   
       6 . The solid support according to  claim 5 , wherein the polypeptide is MDP1 or a polypeptide in which at least one of amino acids making up MDP1 is replaced, added or deleted. 
   
   
       7 . A method for isolating a cell, comprising a step in which the solid support set forth in  claim 1  is contacted with a sample containing a bacterial cell having mycolic acid-containing glycolipid in the cell wall. 
   
   
       8 . A method for isolating a cell, comprising a step in which the solid support set forth in  claim 2  is contacted with a sample containing a bacterial cell having mycolic acid-containing glycolipid in the cell wall; and a step in which solid-liquid separation is carried out by utilizing magnetic action. 
   
   
       9 . The method for isolating a cell according to  claim 7 , wherein the bacterial cell is tuberculosis bacterial cell. 
   
   
       10 . A method for extracting a nucleic acid, comprising a step in which the solid support set forth in  claim 1  is contacted with a sample containing a bacterial cell having mycolic acid-containing glycolipid in the cell wall to isolate the cell; and a step in which a nucleic acid in the isolated bacterial cell is extracted. 
   
   
       11 . A method for extracting a nucleic acid, comprising a step in which the solid support set forth in  claim 2  is contacted with a sample containing a bacterial cell having mycolic acid-containing glycolipid in the cell wall to isolate the cell; a step in which a nucleic acid in the isolated bacterial cell is extracted; and a step in which solid-liquid separation is carried out by utilizing magnetic action. 
   
   
       12 . The method for extracting a nucleic acid according to  claim 10 , wherein the step in which a nucleic acid is extracted further comprises a step in which the cell is lysed by heating, irradiation of ultrasonic wave, or chemical treatment to release a nucleic acid. 
   
   
       13 . A method for isolating a nucleic acid, comprising a step in which the solid support set forth in  claim 3  is contacted with a sample containing a nucleic acid. 
   
   
       14 . A method for isolating a nucleic acid, comprising a step in which the solid support set forth in  claim 4  is contacted with a sample containing a nucleic acid; and a step in which solid-liquid separation is carried out by utilizing magnetic action. 
   
   
       15 . The method for isolating a nucleic acid according to  claim 13 , wherein the nucleic acid has a GC-rich sequence. 
   
   
       16 . A kit comprising a solid support comprising a polypeptide having a capability of binding with mycolic acid-containing glycolipid and/or nucleic acid which is immobilized on the surface of a carrier.