Regeneration

Abstract

The invention relates to the field of regeneration of cells and the vegetative propagation of (micro)-organisms or specific parts such as tissues or organs thereof, for example of those cells grown in tissue or organ culture, and more in particular to the seedless propagation of plants. The invention provides a culture method for propagation of a plant from plant starting material wherein during regeneration of said starting material, especially in the phase of the development of the shoot-root body plan, root or shoot initiation is stimulated by a recombinant gene product or functional fragment thereof, for example derived from a gene involved in the regulation of plant development allowing reducing or omitting exogenous phytohormone addition to said culture.

1 - 31 . (canceled) 
     
     
         32 . A method for propagation of a plant from non-embryogenic plant starting material, the method comprising stimulating root and/or shoot initiation of said plant starting material by having at least one recombinant gene product or functional fragment thereof transiently present in said starting material, wherein a reduction of exogenous phytohormone to said culture as compared with conventional methods is achieved. 
     
     
         33 . A method for propagation of a plant from non-embryogenic plant starting material, the method comprising stimulating root and/or shoot initiation of said plant starting material by having at least one recombinant gene product or functional fragment thereof transiently present in said starting material, and wherein no exogenous phytohormone is added to said culture. 
     
     
         34 . A method according to  claim 32  further comprising transforming a least part of said starting material with a nucleic acid encoding said gene product. 
     
     
         35 . A method according to  claim 32  wherein said culture comprises an in vitro culture. 
     
     
         36 . A method according to  claim 32  wherein said propagation comprises essentially seedless propagation. 
     
     
         37 . A method according to  claim 32  wherein said starting material comprises an individual plant cell, protoplast, explant or plant tissue. 
     
     
         38 . A plant or plant material obtainable by a method according to  claim 32 .