Chromatographic method and system for purifying a botulinum toxin

Abstract

Chromatographic processes and systems for purifying a botulinum toxin from an APF fermentation medium.

1 . A process for purifying a Clostridial toxin comprising the step of sequentially contacting a plurality of chromatography resins with an aqueous medium containing a Clostridial toxin, thereby obtaining a purified Clostridial toxin. 
     
     
         2 . The process for purifying a Clostridial toxin of  claim 1 , wherein the plurality of chromatography resins comprises different chromatography resins. 
     
     
         3 . The process for purifying a Clostridial toxin of  claim 1 , wherein the process is a substantially animal protein free (“APF”) process. 
     
     
         4 . The process for purifying a Clostridial toxin of  claim 1 , wherein the process is an essentially APF process. 
     
     
         5 . The process for purifying a Clostridial toxin of  claim 1 , wherein the process is an APF process. 
     
     
         6 . The process for purifying a Clostridial toxin of  claim 1 , wherein the Clostridial toxin fermentation medium results from a substantially APF fermentation process. 
     
     
         7 . The process for purifying a botulinum toxin of  claim 1 , wherein the Clostridial toxin fermentation medium results from an essentially APF fermentation process. 
     
     
         9 . The process for purifying a botulinum toxin of  claim 1 , wherein the Clostridial toxin fermentation medium results from an APF fermentation process. 
     
     
         10 . A purified Clostridial toxin obtained by the process of  claim 1 .