Method for the production of biomass from plant differentiated tissue

Abstract

The present invention provides a method and a culture medium for the production of food biomass by directly culturing seed kernel tissue, or seed cotyledonary differentiated tissue. The culture medium of the present invention contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose, kinetine, adenine, 2,4 d iclorofenoxiacético (2,4D), L-Glutamine, cysteine, ascorbic acid, and Gelrite®.

1 . A method for the production of biomass from  Sterculiacea  plant differentiated tissue comprising:
 A. Obtaining a fruit that is between three and five months old;   B. Cleaning and sterilizing said fruit;   C. Extracting seeds from said fruit;   D. Discarding the embryo and obtaining a sample of cotyledonary differentiated tissue;   E. Putting the sample of cotyledonary differentiated tissue in contact with culture medium;   F. Incubating under light with a determined wave length, and at a temperature between 15 and 35° C., until biomass is produced; and,   G. Collecting grown biomass by retiring said grown biomass from the culture medium.   
   
   
       2 . The method of  claim 1 , wherein the culture medium contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose in a concentration between 15 and 40 g/L, kinetine in a concentration between 0.5 and 4 mg/L, adenine in a concentration between 0.2 and 5 mg/L, 2,4diclorofenoxiacético (2,4D) in a concentration between 1 and 5 mg/L, L-Glutamine in a concentration between 20 and 100 mg/L, cysteine in a concentration between 10 and 100 mg/L, ascorbic acid in a concentration between 20 and 110 mg/L, and Gelrite® in a concentration between 0.7 and 2.0 g/L; and wherein the pH of the culture medium is adjusted to a range between 5.5 and 6.0. 
   
   
       3 . The method of  claim 1 , wherein the  Sterculiacea  plant is from the  Theobroma  genus. 
   
   
       4 . The method of  claim 1 , wherein the  Sterculiacea  plant is from the  T. cacao  species. 
   
   
       5 . The method of  claim 1 , wherein the sample of cotyledonary differentiated tissue in contact with the culture medium is incubated under total darkness. 
   
   
       6 . A method for the production of biomass from  Proteaceae  plant differentiated tissue comprising:
 a. Obtaining a sample of kernel differentiated tissue;   b. Putting the sample of kernel differentiated tissue in contact with culture medium;   c. Incubating under light with a determined wave length, and at a temperature between 15 and 35° C., until biomass is produced; and,   d. Collecting grown biomass by retiring said grown biomass from the culture medium.   
   
   
       7 . The method of  claim 6 , wherein the culture medium contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose in a concentration between 15 and 40 g/L, kinetine in a concentration between 0.5 and 4 mg/L, adenine in a concentration between 0.2 and 5 mg/L, 2,4diclorofenoxiacético (2,4D) in a concentration between 1 and 5 mg/L, L-Glutamine in a concentration between 20 and 100 mg/L, cysteine in a concentration between 10 and 100 mg/L, ascorbic acid in a concentration between 20 and 110 mg/L, and Gelrite® in a concentration between 0.7 and 2.0 g/L; and wherein the pH of the culture medium is adjusted to a range between 5.5 and 6.0. 
   
   
       8 . The method of  claim 6 , wherein the  Proteaceae  plant is from the  Macadamia  genus. 
   
   
       9 . The method of  claim 6 , wherein the  Proteaceae  plant is from the  Macadamia  species. 
   
   
       10 . The method of  claim 6 , wherein the sample of kernel differentiated tissue in contact with the culture medium is incubated under total darkness.