Human G-Protein Coupled Receptors

Abstract

Two human G-Protein coupled receptor polypeptides and DNA (RNA) encoding each of such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides. Also disclosed are diagnostic methods for detecting a mutation in the nucleic acid sequence of each of the G-protein coupled receptors.

1 . An isolated polynucleotide comprising a polynucleotide having at least 95% identity to a member selected from the group consisting of:
 (a) a polynucleotide encoding a polypeptide comprising amino acids 2 to 342 of SEQ ID NO:2;   (b) a polynucleotide encoding a polypeptide comprising amino acids 1 to 260 of SEQ ID NO:4;   (c) a polynucleotide encoding the same polypeptide encoded by the human cDNA in ATCC™ Deposit No. 209003;   (d) a polynucleotide encoding the same polypeptide encoded by the human cDNA in ATCC™ Deposit No. 209004; and   (e) the complement of (a), (b), (c), or (d).   
     
     
         2 . The isolated polynucleotide of  claim 1  wherein said member is (a). 
     
     
         3 . The isolated polynucleotide of  claim 1  wherein said member is (b). 
     
     
         4 . The isolated polynucleotide of  claim 1  wherein said member is (c). 
     
     
         5 . The isolated polynucleotide of  claim 1  wherein said member is (d). 
     
     
         6 . The isolated polynucleotide of  claim 1  comprising a polynucleotide, which includes nucleotides 226-1251 of SEQ ID NO:1. 
     
     
         7 . The isolated polynucleotide of  claim 1  comprising a polynucleotide, which includes nucleotides 2 to 827 of SEQ ID NO:3. 
     
     
         8 . A method of making a recombinant vector comprising inserting the isolated polynucleotide of  claim 1  into a vector, wherein said polynucleotide is DNA. 
     
     
         9 . A recombinant vector comprising the polynucleotide of  claim 1 , wherein said polynucleotide is DNA. 
     
     
         10 . A recombinant host cell, comprising the recombinant vector of  claim 9 . 
     
     
         11 . A method of producing a host cell, comprising transducing, transforming or transfecting a host cell with the vector of  claim 9 . 
     
     
         12 . A method for producing a polypeptide, comprising:
 (a) culturing the recombinant host cell of  claim 10  under conditions suitable to produce a polypeptide encoded by the polynucleotide; and   (b) recovering the polypeptide from the cell culture.   
     
     
         13 . An isolated polypeptide comprising a mature polypeptide having an amino acid sequence encoded by a polynucleotide which is at least 95% identical to a member selected from the group consisting of:
 (a) a polynucleotide encoding a polypeptide comprising amino acids 2 to 342 of SEQ ID NO:2;   (b) a polynucleotide encoding a polypeptide comprising amino acids 1 to 260 of SEQ ID NO:4; and   (c) the complement of (a) or (b).   
     
     
         14 . An antibody against the polypeptide of  claim 13 . 
     
     
         15 . An antagonist against the polypeptide of  claim 13 . 
     
     
         16 . An agonist against the polypeptide of  claim 13 . 
     
     
         17 . A process for diagnosing a disease condition or a susceptibility to a disease condition in a subject related to the under-expression of the polypeptide of  claim 13  comprising determining a mutation in a nucleic acid sequence encoding said polypeptide.