Compositions comprising viruses and methods for concentrating virus preparations

Abstract

A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

1 - 28 . (canceled) 
   
   
       29 . An adenovirus composition comprising adenovirus particles, sorbitol, and sucrose, wherein the amount of sorbitol and sucrose is sufficient to maintain stability of the adenovirus composition during storage. 
   
   
       30 . The adenovirus composition of  claim 29 , wherein the amount of sorbitol and sucrose is sufficient to maintain the stability of the adenovirus composition when the adenovirus composition is stored at 4° C. 
   
   
       31 . The adenovirus composition of  claim 29 , further comprising Tween 20. 
   
   
       32 . The adenovirus composition of  claim 29 , further comprising Tween 80. 
   
   
       33 . An adenovirus composition comprising adenovirus particles, sorbitol, and Tween 20, wherein the amount of sorbitol and Tween 20 is sufficient to maintain stability of the adenovirus composition during storage. 
   
   
       34 . The adenovirus composition of  claim 31 , wherein the amount of sorbitol and Tween 20 is sufficient to maintain stability of the adenovirus composition when the adenovirus composition is stored at 4° C. 
   
   
       35 . An adenovirus composition comprising adenovirus particles, sorbitol, and Tween 80, wherein the amount of sorbitol and Tween 80 is sufficient to maintain stability of the adenovirus composition during storage. 
   
   
       36 . The adenovirus composition of  claim 35 , wherein the amount of sorbitol and Tween 80 is sufficient to maintain stability of the adenovirus composition when the adenovirus composition is stored at 4° C. 
   
   
       37 . A method for preparing an adenovirus composition, comprising the steps of providing adenovirus particles and combining the adenovirus particles with a solution comprising a buffer, sorbitol, and sucrose, wherein the amount of sorbitol and sucrose is sufficient to maintain stability of the adenovirus composition during storage. 
   
   
       38 . The method of  claim 37 , wherein the amount of sorbitol and sucrose is sufficient to maintain stability of the adenovirus composition when the adenovirus composition is stored at 4° C. 
   
   
       39 . The method of  claim 37 , wherein the adenovirus particles are further combined with Tween 20. 
   
   
       40 . The method of  claim 37 , wherein the adenovirus particles are further combined with Tween 80.