Methods of Identifying Synthetic Transcriptional and Translational Regulatory Elements, and Compositions Related to Same

Abstract

Provided are methods of identifying oligonucleotides having transcriptional or translational activity by integrating ilie oligonucleotide into a eukaryotic cell genome such that the oligonucleotide is operatively linked to an expressible polynucleotide, and detecting a change in expression of the expressible polynucleotide due to the operatively linked oligonucleotide. Also provided are vectors useful for identifying an oligonucleotide having transcriptional or translational regulatory activity according to a method of the invention. In addition, isolated synthetic transcriptional or translational regulatory elements identified according to a method of the invention are provided, as are kits, which contain a vector useful for identifying a transcriptional or translational regulatory element, or an isolated synthetic transcriptional or translational regulatory element or plurality of such elements. Also provided are isolated transcriptional regulatory elements.

1 . An isolated translational regulatory element selected from any of SEQ ID NOS: 102 and 105-108. 
   
   
       2 . The translational regulatory element of  claim 1 , which is an IRES element. 
   
   
       3 . A recombinant nucleic acid molecule comprising a plurality of operatively linked isolated translational regulatory elements of  claim 2 . 
   
   
       4 . The recombinant nucleic acid molecule of  claim 3 , wherein the plurality comprises a plurality of different isolated translational regulatory elements. 
   
   
       5 . An isolated transcriptional regulatory element selected from any of SEQ ID NOS:10, 11, 13, 15 and 15. 
   
   
       6 . A kit, comprising an isolated synthetic translational regulatory oligonucleotide according to  claim 1 . 
   
   
       7 . The kit of  claim 6 , further comprising a vector for containing the oligonucleotide. 
   
   
       8 . The kit of  claim 6 , comprising a plurality of isolated synthetic translational regulatory oligonucleotides.