Particle/cell separation device and compositions

Abstract

A particle/cell separation device is described which is particularly adapted for neutrophil depletion from a preparation of whole blood or platelet-rich plasma. Also described are blood and platelet rich plasma compositions produced using the device which are neutrophil-depleted.

1 . A composition produced by a cell separation device comprising:
 an inlet end portion comprising an inlet port for receiving a sample of platelet rich plasma having a negative electrical charge;   an outlet end portion comprising at least one collection port for removing neutrophil-depleted platelet rich plasma having a positive electrical charge; and   a flow path placed within an electric field comprising a tubular material in fluid communication with the inlet port and the at least one collection port,   
       wherein the tubular material is latticed or coiled and has a diameter of 10-100 microns, and 
       wherein neutrophil content has been reduced by 10% or more. 
     
     
         2 . Neutrophil-depleted whole blood or platelet rich plasma, wherein neutrophil content has been reduced by at least 10% compared to a starting material. 
     
     
         3 . Neutrophil-depleted whole blood or platelet rich plasma, wherein neutrophil content has been reduced by at least 5% compared to a starting material. 
     
     
         4 . A method of preparing neutrophil-depleted platelet rich plasma comprising:
 obtaining a blood sample from a patient;   obtaining platelet rich plasma from the blood sample;   passing the platelet rich plasma through a device for separation of neutrophils from platelet rich plasma to obtain neutrophil-depleted platelet rich plasma, the device comprising:
 an inlet end portion comprising an inlet port for receiving a sample of platelet rich plasma having a negative electrical charge; 
 an outlet end portion comprising at least one collection port for removing neutrophil-depleted platelet rich plasma having a positive electrical charge; and 
   a flow path placed within an electric field comprising a tubular material in fluid communication with the inlet port and the outlet port, wherein the tubular material is latticed or coiled and has a diameter of 10-100 microns; and   collecting the neutrophil-depleted platelet rich plasma.   
     
     
         5 . A method of preparing neutrophil-depleted platelet rich plasma comprising:
 obtaining a blood sample from a patient;   passing the blood sample through a device for separation of neutrophils from platelet rich plasma, the device comprising:
 an inlet end portion comprising an inlet port for receiving a sample of platelet rich plasma having a negative electrical charge; 
 an outlet end portion comprising at least one collection port for removing neutrophil-depleted platelet rich plasma having a positive electrical charge; and 
 a flow path placed within an electric field comprising a tubular material in fluid communication with the inlet port and the at least one collection port, wherein the tubular material is latticed or coiled and has a diameter of 10-100 microns to obtain neutrophil-depleted blood; and 
   processing the neutrophil-depleted blood to obtain neutrophil-depleted platelet rich plasma.   
     
     
         6 . A method of treating an injured tissue in an individual comprising:
 determining a site of tissue injury in the individual; and   introducing a neutrophil-depleted platelet-rich plasma composition into and around the site of tissue injury.   
     
     
         7 . The method of  claim 6 , wherein the tissue is selected from the group consisting of connective tissue, cardiac muscle, skeletal muscle, disc material, a vertebral body, brain, spinal cord, and vascular tissue. 
     
     
         8 . The method of  claim 7 , wherein the tissue is a connective tissue. 
     
     
         9 . The method of  claim 6 , further comprising the step of titrating the neutrophil-depleted platelet-rich plasma to obtain a pH of about 7.3 to 7.5. 
     
     
         10 . The method of  claim 9 , wherein the titration is performed using a bicarbonate buffer. 
     
     
         11 . The method of  claim 6 , wherein the neutrophil-depleted platelet-rich plasma composition comprises platelets obtained from the individual. 
     
     
         12 . The method of  claim 6 , wherein no exogenous activator is added to the composition prior to its introduction into and around the site of injury. 
     
     
         13 . The method of  claim 6 , further comprising the step of mixing into the neutrophil-depleted platelet composition substantially simultaneously with the introduction into and around the site of tissue injury, one or more ingredients selected from the group consisting of thrombin, epinephrine, collagen, calcium salts, and pH adjusting agents. 
     
     
         14 . A neutrophil-depleted platelet-rich plasma composition for the treatment of an injured tissue comprising:
 neutrophil-depleted platelet-rich plasma; and   a pH adjusting agent,   
       wherein the neutrophil-depleted platelet-rich plasma composition does not contain an activator of the neutrophil-depleted platelet-rich plasma. 
     
     
         15 . The neutrophil-depleted platelet-rich plasma composition of  claim 14 , wherein the pH adjusting agent provides a pH of about 7.3 to 7.5. 
     
     
         16 . The neutrophil-depleted platelet-rich plasma composition of  claim 14 , wherein the pH adjusting agent is a bicarbonate buffer. 
     
     
         17 . The neutrophil-depleted platelet-rich plasma composition of  claim 14 , wherein a plasma used for the neutrophil-depleted platelet-rich plasma is from an autologous source.