Angiostatin Fragments and Method of Use

Abstract

Fragments of an endothelial cell proliferation inhibitor and method of use therefor are provided. The endothelial proliferation inhibitor is a protein derived from plasminogen, or more specifically is an angiostatin fragment. The angiostatin fragments generally correspond to kringle structures occurring within the endothelial cell proliferation inhibitor. The endothelial cell inhibiting activity of these fragments provides a means for inhibiting angiogenesis of tumors and for treating angiogenic-mediated disease.

1 - 63 . (canceled) 
     
     
         64 . An isolated nucleic acid molecule consisting of a nucleotide sequence encoding a fragment of plasminogen,
 wherein the fragment of plasminogen consists of a kringle 2-3 fragment, a kringle 1-3 fragment, a kringle 1-2 fragment, a kringle 4 fragment, a kringle 1-4 fragment, or a kringle 1-4BKLS fragment.   
     
     
         65 . A vector comprising a nucleotide sequence encoding a fragment of plasminogen, wherein
 the fragment of plasminogen consists of a kringle 2-3 fragment, a kringle 1-3 fragment, a kringle 1-2 fragment, a kringle 4 fragment, a kringle 1-4 fragment, or a kringle 1-4BKLS fragment, and   when the vector is expressed, the fragment of plasminogen is produced.   
     
     
         66 . A method of modulating angiogenesis in an animal or a human comprising administering to the animal or the human a composition comprising a nucleotide sequence encoding a fragment of plasminogen, wherein
 the fragment of plasminogen consists of a kringle 2-3 fragment, a kringle 1-3 fragment, a kringle 1-2 fragment, a kringle 4 fragment, a kringle 1-4 fragment, or a kringle 1-4BKLS fragment, and   when the vector is expressed, the fragment of plasminogen is produced.