Composite expandable device with impervious polymeric covering and bioactive coating thereon, delivery apparatus and method

Abstract

A composite expandable device for delivery into a vessel carrying blood comprising an expandable support frame having first and second end portions. An impervious polymer sleeve having inner and outer surfaces extending over the support frame. A coating is disposed on at least one of the inner and outer surfaces of the polymer sleeve for enhancing endothelial cell growth on the device and polymer sleeve.

1 . An expandable device for delivery into a blood vessel carrying blood comprising:
 an expandable support frame having first and second end portions,   a polymer sleeve having inner and outer surfaces and where the polymer sleeve comprises a polymer that is difficult to obtain endothelial cell growth thereon, and a coating having a first layer capable of providing free amine groups comprising a plasma activated stable functional group, a second linker layer having a terminal COOH group, and a third cell adhesion peptide layer, wherein the linker layer is positioned between and covalently bonded to each of the first and third layers, said coating carried on and attached to at least one of the inner and outer surfaces of the polymer sleeve for enhancing endothelial cell growth on the polymer sleeve, wherein second linker layer coverage with linkers having a terminal COOH group reduces a thrombogenic risk of unbound linkers on the layer.   
     
     
         2 . The device of  claim 1 , wherein said coating is prepared by treating said inner and outer surface with a gaseous plasma cleaning process utilizing radiofrequency energy to ablate said inner or outer surface and to functionalize said inner or outer surface and to produce a plasma-deposited layer having functional groups, and
 subjecting said plasma-deposited layer to multifunctional linkers in a wet chemical treatment to form covalent bonds between the linkers/spacers and the functional groups of the plasma-deposited layer to covalently bind the cell-adhesion peptides to said inner or outer surface of the substrate.   
     
     
         3 . The device of  claim 1 , wherein said cell-adhesion peptide has an amino acid sequence presented as SEQ ID NO: 1. 
     
     
         4 . The device of  claim 2 , wherein said cell-adhesion peptide has an amino acid sequence presented as SEQ ID NO: 1. 
     
     
         5 . The device of  claim 1 , wherein a linker having a terminal COOH group binds one or more cell-adhesion peptides having an amino acid sequence presented as SEQ ID NO: 1. 
     
     
         6 . The device of  claim 2 , wherein a linker having a terminal COOH group binds one or more cell-adhesion peptides having an amino acid sequence presented as SEQ ID NO: 1.