Pichia methanolica secretory signal

Abstract

Novel Pichia methanolica secretory signal polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods of using are disclosed. Methods of producing large amounts of recombinant proteins by employing DNA constructs having a polypeptide of interest preceded by a novel Pichia methanolica secretory signal sequence.

1 . A fusion protein comprising a first portion and a second portion joined by a peptide bond, wherein the first portion comprises an amino acid sequence of SEQ ID NO:2, and the second portion comprises another polypeptide. 
     
     
         2 . The fusion protein of  claim 1  wherein the another polypeptide of the second portion comprises an antibody or antibody fragment. 
     
     
         3 . A fusion protein comprising a first portion, a second portion, and a third portion, wherein the first portion is joined by a peptide bond to the second portion and the second portion is joined by a peptide bond to the third portion, wherein the first portion comprises an amino acid sequence of SEQ ID NO:2, the second portion comprises another polypeptide, and the third portion comprises an affinity tag, a therapeutic agent or a detectable label. 
     
     
         4 . The fusion protein of  claim 3  wherein the another polypeptide of the second portion comprises an antibody or antibody fragment. 
     
     
         5 . A fusion protein comprising a first portion, a second portion, and a third portion, wherein the first portion is joined by a peptide bond to the second portion and the second portion is joined by a peptide bond to the third portion, wherein the first portion comprises an amino acid sequence of SEQ ID NO:2, the second portion comprises another polypeptide, and the third portion comprises an immunoglobulin moiety. 
     
     
         6 . The fusion protein of  claim 5  wherein the another polypeptide of the second portion comprises an antibody or antibody fragment. 
     
     
         7 . The fusion protein of  claim 5  wherein the immunoglobulin moiety of the third portion comprises an immunoglobulin Fc fragment.