US2009028921A1PendingUtilityA1

Electrospun Ceramic-Polymer Composite As A Scaffold For Tissue Repair

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Assignee: NEW JERSEY TECH INSTPriority: Jun 18, 2007Filed: Jun 18, 2008Published: Jan 29, 2009
Est. expiryJun 18, 2027(~0.9 yrs left)· nominal 20-yr term from priority
Inventors:Treena Arinzeh
A61F 2/28D01F 1/10D01D 5/0007A61L 27/3834A61F 2/3094A61F 2002/4495A61F 2310/00179A61F 2240/001A61L 27/3847A61L 27/46A61L 27/3821
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Claims

Abstract

The present invention relates to compositions and methods of preparing a three-dimensional matrix of micron sized electrospun fibers, wherein the electrospun fibers are formed from a electrospun composite comprising a bioactive ceramic component and a polymer component. The matrix provides an osteoconductive and osteoinductive scaffold supporting osteogenesis and thereby facilitates bone repair.

Claims

exact text as granted — not AI-modified
1 . An implantable scaffold for use in tissue engineering or as an implantable material comprising
 a three-dimensional matrix of micron sized electrospun fibers,   wherein the electrospun fibers are formed from an electrospun composite comprising a bioactive ceramic component and a polymer component.   
     
     
         2 . The implantable scaffold according to  claim 1 , wherein the electrospun composite contains at least 10% by weight of the ceramic component. 
     
     
         3 . The implantable scaffold according to  claim 1 , wherein the electrospun composite contains at least about 60% by weight of the polymer component. 
     
     
         4 . The implantable scaffold according to  claim 1 , wherein the electrospun composite contains at least 10% by weight of the ceramic component and at least about 60% by weight of the polymer component. 
     
     
         5 . The implantable scaffold according to  claim 1 , wherein the bioactive ceramic component contains two calcium phosphate ceramic substances for every polymer of the polymer component of the composite. 
     
     
         6 . The implantable scaffold according to  claim 5 , wherein the calcium phosphate ceramic substances of the bioactive ceramic component of the electrospun composite are selected from the group consisting of tetracalcium phosphate, amorphous calcium phosphate, alpha-tricalcium phosphate, beta-tricalcium phosphate, and hydroxyapatite. 
     
     
         7 . The implantable scaffold according to  claim 5 , wherein the two calcium phosphate ceramic substances of the bioactive ceramic component of the electrospun composite are hydroxyapatite and tricalcium phosphate. 
     
     
         8 . The implantable scaffold according to  claim 7 , wherein the bioactive ceramic component of the electrospun composite comprises 20% hydroxyapatite and 80% tricalcium phosphate. 
     
     
         9 . The implantable scaffold according to  claim 1 , wherein the polymer component of the electrospun composite is at least one polymer selected from the group consisting of a nondegradable polymer and a degradable polymer. 
     
     
         10 . The implantable scaffold according to  claim 9 , wherein the nondegradable polymer is selected from the group consisting of a polyurethane, a polyvinylidine fluoride, and a polyvinylidine fluoride trifluoroethylene. 
     
     
         11 . The implantable scaffold according to  claim 9 , wherein the degradable polymer is selected from the group consisting of a poly(lactic acid-glycolic acid), a poly(lactic acid), a poly(glycolic acid), a poly(orthoester), a poly(phosphazene), poly(or polycaprolactone, a polyamide, a polysaccharide, and a collagen. 
     
     
         12 . The implantable scaffold according to  claim 1 , wherein the polymer of the polymer component of the electrospun composite is polycaprolactone. 
     
     
         13 . The implantable scaffold according to  claim 1 , wherein the three dimensional matrix of electrospun fibers comprises micron-sized pores. 
     
     
         14 . A composition for preparing an implantable osteoinductive and osteoconductive three-dimensional matrix of micron sized electrospun fibers, the composition comprising an electrospun composite containing a bioactive ceramic component and a polymer component. 
     
     
         15 . The composition according to  claim 14 , wherein the electrospun composite of the composition contains at least 10% by weight of the ceramic component. 
     
     
         16 . The composition according to  claim 14 , wherein the electrospun composite of the composition contains at least about 60% by weight of the polymer component. 
     
     
         17 . The composition according to  claim 14 , wherein the electrospun composite of the composition contains at least 10% by weight of the ceramic component and at least about 60% by weight of the polymer component of the composite. 
     
     
         18 . The composition according to  claim 14 , wherein the bioactive ceramic component of the electrospun composite of the composition contains two calcium phosphate ceramic substances for every polymer in the polymer component. 
     
     
         19 . The composition according to  claim 18 , wherein the calcium phosphate ceramic substances of the bioactive ceramic component of the electrospun composite of the composition are selected from the group consisting of tetracalcium phosphate, amorphous calcium phosphate, alpha-tricalcium phosphate, beta-tricalcium phosphate, and hydroxyapatite. 
     
     
         20 . The composition according to  claim 18 , wherein the two calcium phosphate ceramic substances of the bioactive ceramic component of the electrospun composite of the composition are hydroxyapatite and tricalcium phosphate. 
     
     
         21 . The composition according to  claim 20 , wherein the bioactive ceramic component of the electrospun composite of the composition comprises 20% hydroxyapatite and 80% tricalcium phosphate. 
     
     
         22 . The composition according to  claim 14 , wherein the polymer component of the electrospun composite of the composition is at least one polymer selected from the group consisting of a nondegradable polymer and a degradable polymer. 
     
     
         23 . The composition according to  claim 22 , wherein the nondegradable polymer is selected from the group consisting of a polyurethane, a polyvinylidine fluorides and a polyvinylidine fluoride trifluoroethylene. 
     
     
         24 . The composition according to  claim 22 , wherein the degradable polymer is selected from the group consisting of poly(lactic acid-glycolic acid), poly(lactic acid), poly(glycolic acid), a poly(orthoester), a poly(phosphazene), a polycaprolactone, a polyamide, a polysaccharide, and a collagen. 
     
     
         25 . The composition according to  claim 14 , wherein the polymer of the polymer component of the electrospun composite of the composition is polycaprolactone. 
     
     
         26 . A method of preparing an osteoinductive and osteoconductive scaffold to facilitate bone repair, the method comprising the steps:
 (a) preparing a ceramic-polymer composite comprising a bioactive ceramic component and a polymer component;   (b) electrospinning the ceramic-polymer composite, and   (c) thereby depositing a three-dimensional nonwoven matrix of electrospun fibers comprising the ceramic-polymer composite on a collector.   
     
     
         27 . The method according to  claim 26 , wherein the electrospun composite of step (a) contains at least 10% by weight of the ceramic component. 
     
     
         28 . The method according to  claim 26 , wherein the electrospun composite of step (a) contains at least about 60% by weight of a polymer component. 
     
     
         29 . The method according to  claim 26 , wherein the electrospun composite of step (a) contains at least 10% by weight of the ceramic component and at least about 60% by weight of the polymer component. 
     
     
         30 . The method according to  claim 26 , wherein the bioactive ceramic component of the composite of step (a) contains two calcium phosphate ceramic substances for every polymer in the polymer component of the composite. 
     
     
         31 . The method according to  claim 30 , wherein the calcium phosphate ceramic substances of the bioactive ceramic component of the composite of step (a) are selected from the group consisting of tetracalcium phosphate, amorphous calcium phosphate, alpha-tricalcium phosphate, beta-tricalcium phosphate, and hydroxyapatite. 
     
     
         32 . The method according to  claim 30 , wherein the two calcium phosphate ceramic substances of the bioactive ceramic component of the composite of step (a) are hydroxyapatite and tricalcium phosphate. 
     
     
         33 . The method according to  claim 32 , wherein the bioactive ceramic component of the composite of step (a) comprises 20% hydroxyapatite and 80% tricalcium phosphate. 
     
     
         34 . The method according to  claim 26 , wherein the polymer component of the composite of step (a) is at least one polymer selected from the group consisting of a nondegradable polymer and a degradable polymer. 
     
     
         35 . The method according to  claim 34 , wherein the nondegradable polymer is selected from the group consisting of a polyurethane, a polyvinylidine fluoride, and a polyvinylidine fluoride trifluoroethylene. 
     
     
         36 . The method according to  claim 34 , wherein the degradable polymer is selected from the group consisting of poly(lactic acid-glycolic acid), poly(lactic acid), poly(glycolic acid), a poly(orthoester), a poly(phosphazene), a polycaprolactone, a polyamide, a polysaccharide, and a collagen. 
     
     
         37 . The method according to  claim 26 , wherein the polymer of the polymer component of the composite of step (a) is polycaprolactone. 
     
     
         38 . The method according to  claim 26 , wherein the electrospun fibers of step (c) are micron sized. 
     
     
         39 . The method according to  claim 26 , wherein the matrix of electrospun fibers in step (c) comprises micron-sized pores. 
     
     
         40 . The method according to  claim 26 , further comprising the steps:
 (d) seeding the three-dimensional nonwoven matrix of electrospun fibers with isolated differentiable human mesenchymal cells or osteoblasts; and   (e) growing the differentiable human mesenchymal cells or osteoblasts on the three-dimensional nonwoven matrix of electrospun fibers so that the differentiable human mesenchymal cells or osteoblasts differentiate into a mature cell phenotype on the scaffold.

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