Gel having biosubstance fixed thereto and microarray utilizing the gel

Abstract

The present invention provides a biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel.

1 - 7 . (canceled) 
     
     
         8 . A gel-filled hollow tube which comprises a hollow tube whose hollow space is filled with the biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel. 
     
     
         9 . The gel-filled hollow tube according to  claim 8 , wherein the hollow tube is a hollow fiber. 
     
     
         10 . A method for manufacturing a biological substance-immobilized gel microarray, which comprises allowing a plurality of gel-filled hollow tubes according to  claim 8  to be tied in a bundle and cutting the resulting tube bundle in a direction intersecting with the longitudinal direction of the tubes. 
     
     
         11 . A method for manufacturing a biological substance-immobilized gel microarray, which comprises the following steps:
 (1) allowing a plurality of hollow tubes to be tied in a bundle;   (2) filling the biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel.   into the hollow space of each tube in the resulting tube bundle; and   (3) cutting the tube bundle in a direction intersecting with the longitudinal direction of the tubes.   
     
     
         12 . A biological substance-immobilized gel microarray which comprises the biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel, wherein the gel is arranged in multiple compartments. 
     
     
         13 . The biological substance-immobilized gel microarray according to  claim 12 , wherein the surface area of each compartment is 10 −6  m 2  or less. 
     
     
         14 . The biological substance-immobilized gel microarray according to  claim 12 , wherein the compartments are formed by slots or through holes. 
     
     
         15 . A biological substance-immobilized gel microarray which is obtained by allowing a plurality of gel-filled hollow tubes according to  claim 8  to be tied in a bundle and cutting the tube bundle in a direction intersecting with the longitudinal direction of the tubes. 
     
     
         16 . The biological substance-immobilized gel microarray according to  claim 15 , wherein the hollow tubes are hollow fibers. 
     
     
         17 - 19 . (canceled) 
     
     
         20 . The gel-filled hollow tube according to  claim 8 , which comprises a gel having the following composition and a biological substance immobilized on and/or in the gel: 
       
         
           
                 
                 
                 
                 
               
                     
                     
                 
                     
                   (a) N,N-dimethylacrylamide 
                   2% to 7% 
                   by mass 
                 
                     
                   (b) cross-linking agent 
                   0.1% to 1.5% 
                   by mass. 
                 
                     
                     
                 
             
                
               
               
                
                
                
               
            
           
         
       
     
     
         21 . The gel-filled hollow tube according to  claim 8 , wherein the biological substance is a nucleic acid. 
     
     
         22 . The gel-filled hollow tube according to  claim 20 , wherein the cross-linking agent is a multifunctional monomer having at least two ethylenically unsaturated bonds. 
     
     
         23 . The biological substance-immobilized gel according to  claim 22 , wherein the cross-linking agent is methylenebisacrylamide.