Method of retrovirus storage

Abstract

A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.

1 . A method for storing a retrovirus, the method comprising maintaining a retrovirus in the presence of a substance having a retrovirus-binding activity immobilized on a solid support. 
   
   
       2 . The method according to  claim 1 , wherein the retrovirus is maintained in an unfrozen state. 
   
   
       3 . The method according to  claim 1 , wherein the retrovirus is maintained in a solution so that the solution is not in contact with air. 
   
   
       4 . The method according to  claim 1 , wherein the retrovirus is maintained being separated from other retrovirus producer cell-derived components. 
   
   
       5 . The method according to  claim 4 , wherein the retrovirus is maintained in a buffer containing a phosphate salt as a buffering component. 
   
   
       6 . The method according to  claim 4 , wherein the retrovirus is maintained in a solution containing a substance selected from the group consisting of proteins and saccharides. 
   
   
       7 . The method according to  claim 1 , wherein the substance having a retrovirus-binding activity is a substance selected from the group consisting of a polypeptide having the heparin-II domain of fibronectin, fibroblast growth factor, a polypeptide having the insulin-binding domain of type V collagen, DEAE-dextran and polylysine. 
   
   
       8 . A composition containing a retrovirus, wherein the composition is contained in a container carrying a solid support on which a substance having a retrovirus-binding activity is immobilized, and the retrovirus is bound to the substance having a retrovirus-binding activity. 
   
   
       9 . The composition according to  claim 8 , wherein the retrovirus is contained in a solution in the container so that the solution is not in contact with air. 
   
   
       10 . The composition according to  claim 8 , wherein the retrovirus is contained in the container being separated from other retrovirus producer cell-derived components. 
   
   
       11 . The composition according to  claim 10 , which further contains a buffer containing a phosphate salt as a buffering component. 
   
   
       12 . The composition according to  claim 10 , which further contains a substance selected from the group consisting of proteins and saccharides in a solution. 
   
   
       13 . The composition according to  claim 8 , wherein the substance having a retrovirus-binding activity is a substance selected from the group consisting of a polypeptide having the heparin-II domain of fibronectin, fibroblast growth factor, a polypeptide having the insulin-binding domain of type V collagen, DEAE-dextran and polylysine.