Polypeptides having Lipase Activity and Polynucleotides Encoding Same

Abstract

The present invention relates to polypeptide having lipase activity and which further has a RP of at least 0.8 and a BR of at least 1.1 at the test conditions given in the specification.

1 . A polypeptide having lipase activity and which further has an Average Relative Performance (RPavg) of at least 0.8 and a Benefit Risk (BR) of at least 1.1 at the test conditions given in the specification. 
     
     
         2 . The polypeptide of  claim 1 , which further has a relative LU/A280 less than 1.00 at the test conditions given in the specification. 
     
     
         3 . The polypeptide of  claim 1  which is a bacterial polypeptide. 
     
     
         4 . The polypeptide of  claim 1  which is a fungal polypeptide. 
     
     
         5 . The polypeptide of  claim 4  which is a  Thermomyces  polypeptide. 
     
     
         6 . The polypeptide of  claim 5  which is a  Thermomyces lanuginosus  polypeptide. 
     
     
         7 . The polypeptide of  claim 1  which is a variant of a lipase comprised by the polypeptide of SEQ ID NO: 2. 
     
     
         8 . The polypeptide of  claim 1  which is a variant of a lipase comprised by the mature part of the polypeptide of SEQ ID NO: 2. 
     
     
         9 . The polypeptide of  claim 1  which is a variant of a lipase comprising the polypeptide of SEQ ID NO: 2. 
     
     
         10 . The polypeptide of  claim 1  which is a variant of a lipase comprising the mature part of the polypeptide of SEQ ID NO: 2. 
     
     
         11 . The polypeptide of  claim 1 , which is encoded by a polynucleotide which hybridizes under at least high stringency conditions with nucleotides 644 to 732 of SEQ ID NO: 1 or a complementary strand hereto. 
     
     
         12 . An isolated polynucleotide comprising a nucleotide sequence which encodes the polypeptide of any of the preceding claims. 
     
     
         13 . A nucleic acid construct comprising the polynucleotide of  claim 12  operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 
     
     
         14 . A recombinant expression vector comprising the nucleic acid construct of  claim 13 . 
     
     
         15 . A recombinant host cell comprising the nucleic acid construct of  claim 14 . 
     
     
         16 . A method for producing the polypeptide of  claim 1  comprising
 (a) cultivating a cell, which in its wild-type form is capable of producing the polypeptide, under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         17 . A method for producing the polypeptide of  claim 1  comprising
 (a) cultivating a host cell comprising a nucleic acid construct comprising a nucleotide sequence encoding the polypeptide under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.