Defective sindbis viral vectors

Abstract

Disclosed herein are new defective Sindbis viral vectors made from wild type Ar-339 Sindbis virus, with differences in replicase and envelope proteins between JT vectors and consensus Sindbis virus sequences, and also between JT and Ar-339 vectors. Also disclosed are plasmids used for the production of the vectors, methods for producing the vectors, methods for treating mammals suffering from tumors and pharmaceutical formulations for use in the treatment methods.

1 . A purified, isolated nucleic acid comprising the nucleotide sequence as set forth in nucleotides 1-4831 of SEQ ID NO.: 36. 
     
     
         2 . The nucleic acid of  claim 1 , further comprising a bacteriophage promoter. 
     
     
         3 . The nucleic acid of  claim 2 , further comprising a bacterial antibiotic resisitance gene. 
     
     
         4 . The nucleic acid of  claim 3  wherein said promoter is the T7 promoter. 
     
     
         5 . The nucleic acid of  claim 3  wherein said promoter is the SP6 promoter. 
     
     
         6 . A purified, isolated nucleic acid comprising the nucleotide sequence as set forth in nucleotides 1-8048 as set forth in SEQ ID NO.: 39. 
     
     
         7 . The nucleic acid of  claim 6 , further comprising a bacteriophage promoter. 
     
     
         8 . The nucleic acid of  claim 7 , further comprising a bacterial antibiotic resistance gene. 
     
     
         9 . The nucleic acid of  claim 7  wherein said promoter is the T7 promoter. 
     
     
         10 . The nucleic acid of  claim 7  wherein said promoter is the SP6 promoter. 
     
     
         11 . A purified, isolated nucleic acid comprising a nucleic acid consisting of nucleotides 1-4831 as set forth in SEQ ID NO.: 36. 
     
     
         12 . A purified, isolated nucleic acid comprising a nucleotide sequence consisting of nucleotides 1-4831 as set forth in SEQ ID NO.: 36 and a bacteriophage promoter. 
     
     
         13 . A purified, isolated nucleic acid comprising a nucleotide sequence consisting of nucleotides 1-4831 as set forth in SEQ ID NO.: 36, a bacteriophage promoter and a bacterial antibiotic resistance gene. 
     
     
         14 . A purified, isolated nucleic acid comprising a nucleotide sequence consisting of nucleotides 1-8048 as set forth in SEQ ID NO.: 39. 
     
     
         15 . A purified, isolated nucleic acid comprising a nucleotide sequence consisting of nucleotides 1-8048 as set forth in SEQ ID NO.: 39 and a bacteriophage promoter. 
     
     
         16 . A purified, isolated nucleic acid comprising a nucleotide sequence consisting of nucleotides 1-8048 as set forth in SEQ ID NO.: 39, a bacteriophage promoter and a bacterial antibiotic resistance gene.