Cosmetic preparation and method for preparing the same

Abstract

The present invention relates to the use of dedifferentiated plant cells in cosmetic preparations for protecting of stem cells against intrinsic and extrinsic stress factors, in particular for promoting proliferation of stem cells and for protecting them against apoptosis. In particular, the invention relates to the use of dedifferentiated plant cells from fruits of Malus domestica (Apple) cultivar Uttwiler Spaetlauber. Further, the invention relates to a method for cultivating of dedifferentiated plant cells, as well as to the preparation of extracts of plant cell cultures which are suitable for such applications.

1 . A cosmetic preparation comprising at least one active component protecting stem cells against intrinsic and extrinsic stress factors, said active component originating from a dedifferentiated vegetable stem cell suspension. 
   
   
       2 . A cosmetic preparation as set forth in  claim 1 , wherein said least one active component promotes proliferation of stem cells. 
   
   
       3 . A cosmetic preparation as set forth in  claim 1 , wherein said least one active component protects stem cells against apoptosis. 
   
   
       4 . A cosmetic preparation as set forth in  claim 1 , wherein said stem cells which are to be protected are skin stem cells. 
   
   
       5 . A cosmetic preparation as set forth in  claim 1 , wherein said dedifferentiated vegetable stem cells which are to be protected are hair follicle stem cells. 
   
   
       6 . A cosmetic preparation as set forth in  claim 1 , wherein said dedifferentiated vegetable stem cells originate from plants of the family of Rosaceae. 
   
   
       7 . A cosmetic preparation as set forth in  claim 6 , wherein said dedifferentiated vegetable stem cells originate from plants of the sub-family of Maloidae. 
   
   
       8 . A cosmetic preparation as set forth in  claim 7 , wherein said dedifferentiated vegetable stem cells originate from plants of  Malus domestica  cultivar Uttwiler Spaetlauber. 
   
   
       9 . A cosmetic preparation as set forth in  claim 1 , wherein said dedifferentiated vegetable stem cell suspension is obtained by high pressure homogenization with liposomes. 
   
   
       10 . A cosmetic preparation as set forth in  claim 1 , comprising 0.01 to 100 percent by weight of dedifferentiated vegetable stem cell suspension. 
   
   
       11 . A cosmetic preparation as set forth in  claim 10 , comprising 0.1 to 10 percent by weight of dedifferentiated vegetable stem cell suspension. 
   
   
       12 . A method of preparing a dedifferentiated vegetable stem cell suspension suitable for use in a cosmetic preparation as set forth in one of the preceding claims, comprising the following procedure:
 decomposition of the plant cells by high pressure homogenization; and   extraction and stabilization of the ingredients by means of liposomes;   
     wherein both parts of the method are simultaneously carried out in a single step.