Device for electrophoresis, device for transfer, device for electrophoresis and transfer, chip for electrophoresis and transfer, and method for electrophoresis, method for transfer, and method for electrophoresis and transfer

Abstract

A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.

1 . A device for electrophoresis configured to separate separation target components in a medium in which a plurality of electric conductors are provided in contact with the medium, comprising:
 voltage applying means configured to apply a voltage to the medium, so that a potential difference between adjacent electric conductors is more than 0V but not more than 0.3V.   
     
     
         2 . The device for electrophoresis as set forth in  claim 1 , wherein:
 the voltage applying means applies the voltage to each of the electric conductors.   
     
     
         3 . The device for electrophoresis as set forth in  claim 1 , wherein:
 the electric conductor is line-shaped.   
     
     
         4 . The device for electrophoresis as set forth in  claim 3 , wherein:
 the line-shaped electric conductors are arranged in parallel one another and extend in an orthogonal direction to a direction in which the voltage applying means applies the voltage to the medium.   
     
     
         5 . A device for electrophoresis and transfer comprising:
 first voltage applying means configured to apply a voltage with respect to a specific direction in a first medium including separation target components; and   second voltage applying means configured to apply a voltage to the first medium toward a direction of a second medium being contact with the first medium,   the second voltage applying means including a first electrode having a plurality of electrode regions being insulated one another and arranged in the specific direction.   
     
     
         6 . The device for electrophoresis and transfer as set forth in  claim 5 , wherein:
 the electrode region is line-shaped.   
     
     
         7 . The device for electrophoresis and transfer as set forth in  claim 6 , wherein:
 the plurality of electrode regions are arranged in parallel one another and extend in an orthogonal direction to the specific direction.   
     
     
         8 . The device for electrophoresis and transfer as set forth in  claim 5  further comprising:
 wire connection means configured to switch the first electrode between connection and disconnection.   
     
     
         9 . The device for electrophoresis and transfer as set forth in  claim 5 , wherein:
 the second voltage applying means further includes a detachable second electrode.   
     
     
         10 . The device for electrophoresis and transfer as set forth in  claim 9  further comprising:
 a detachable holder configured to hold the second electrode and the second medium.   
     
     
         11 . The device for electrophoresis and transfer as set forth in  claim 5 , wherein:
 the second voltage applying means includes a second electrode having a plurality of electrode regions being insulated one another and arranged in the specific direction.   
     
     
         12 . A chip for electrophoresis and transfer comprising:
 a separation section configured to place a first medium including separation target components, and a second medium in contact with the first medium;   a first buffer solution chamber and a second buffer solution chamber sandwiching the separation section; and   a first electrode being provided on the separation section, and including a plurality of electrode regions being insulated one another and arranged in a specific direction specified by the first buffer solution chamber and the second buffer solution chamber.   
     
     
         13 . A method for electrophoresis and transfer comprising:
 (a) applying a voltage to a first medium including separation target components in a specific direction; and   (b) applying a voltage to the first medium toward a direction of the second medium being in contact with the first medium, following the step (a),   wherein in the step (b), the voltage is applied with use of a first electrode including a plurality of electrode regions being insulated one another and arranged in parallel one another in the specific direction.   
     
     
         14 . A device for transfer configured to transfer transfer target components in a first medium to a second medium comprising:
 voltage applying means configured to apply a voltage to the first medium;   the voltage applying means applying the voltage to the first medium in such a manner that a certain position and another position in the first medium are provided different voltages or different voltage durations.   
     
     
         15 . The device for transfer as set forth in  claim 14 , wherein:
 the voltage applying means increases the voltage stepwise or gradually toward a specific direction specified by the first medium.   
     
     
         16 . The device for transfer as set forth in  claim 14 , wherein:
 the voltage applying means increases an applied voltage duration stepwise or gradually toward a specific direction specified by the first medium.   
     
     
         17 . The device for transfer as set forth in  claim 14 , wherein:
 the voltage applying means includes a first electrode and a second electrode, each of which is divided into a plurality of electrode regions being insulated one another.   
     
     
         18 . The device for transfer as set forth in  claim 17 , wherein:
 the electrode region is line-shaped.   
     
     
         19 . The device for transfer as set forth in  claim 18 , wherein:
 the electrode regions are arranged in parallel one another and extend in an orthogonal direction to the specific direction.   
     
     
         20 . The device for transfer as set forth in  claim 17 , wherein:
 the voltage applying means applies the voltage to each of the electric regions.   
     
     
         21 . The device for transfer as set forth in  claim 20 , wherein:
 the voltage applying means comprises at least one power supply, and a plurality of conductive paths connecting the power supply and each of the electrode regions conductively, and   the conductive paths have at least two kinds of resistance values.   
     
     
         22 . The device for transfer as set forth in  claim 20 , wherein:
 the voltage applying means alters a potential duration applied to each of the electrode regions.   
     
     
         23 . The device for transfer as set forth in  claim 22 , wherein:
 the voltage applying means comprises a power supply and a mobile electric conductive section conductively connecting the power supply and the plurality of conductive regions, and   the mobile electric conductive section alters duration of potential application to each of the electrode regions by its movement.   
     
     
         24 . The device for transfer as set forth in  claim 14 , wherein:
 the voltage applying means applies the voltage to the first medium via an electric resistance layer, and   a certain position and another position on the electric resistance layer have different resistance values.   
     
     
         25 . A method for transfer for transferring transfer target components in a first medium to a second medium, comprising:
 applying a voltage to the first medium, in such a manner that a certain position and another position in the first medium are provided different voltages or different voltage durations.