Compositions comprising viruses and methods for concentrating virus preparations

Abstract

A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

1 - 28 . (canceled) 
     
     
         29 . A purified recombinant adenovirus composition obtained by a method of purifying a virus preparation, the method comprising the steps of:
 a) subjecting the virus preparation to anion-exchange chromatography, wherein the virus is eluted as a virus preparation product from an anion-exchange chromatographic medium;   b) adding a polyhydroxy hydrocarbon to the virus preparation product of step (a) so that the concentration of polyhydroxy hydrocarbon in the product reaches a level of about 25% or more; and   c) increasing the concentration of virus in the product of step (b) by applying pressure to the product such that diluent is removed from the product through a filter while the virus is retained,   wherein the composition has a virus concentration of about 1×10 9  to 1×10 13  particles/ml.   
     
     
         30 . The composition of  claim 29 , having a virus titer of about 5.8×10 8  to about 3.6×10 9  pfu/ml. 
     
     
         31 . The composition of  claim 29 , having a virus concentration of about 1×10 9  to about 1×10 12  particles/ml. 
     
     
         32 . The composition of  claim 31 , having a virus concentration of about 1×10 10  to about 1×10 12  particles/ml.