Novel Gonadotropin-Releasing Hormone, Precursor Peptides Thereof and Genes Encoding the Same
Abstract
A novel peptide found as gonadotropin-releasing hormone (GnRH) in octopuses, a member of mollusks. The structure and the activities of the peptide have been unraveled. The peptide finds use as a reagent used in the studies of correlations between the structure and the activities of GnRH and the information processing mechanisms of the nervous systems in higher animals. It can also serve as a base compound in the development of pesticides and drugs. The peptide has the GnRH activity and has the following amino acid sequence (I): (I) <Glu-Zaa-Zaa-His-Zaa-Ser-Zaa-Zaa-Zaa-Zaa-Pro- Gly-NH 2 wherein <Glu represents pyroglutamic acid and Zaa represents any amino acid. Specifically, the peptide has the following amino acid sequence (1): (1) <Glu-Asn-Tyr-His-Phe-Ser-Asn-Gly-Trp-His-Pro-Gly- NH 2 wherein <Glu represents pyroglutamic acid.
Claims
exact text as granted — not AI-modified1 . A peptide having the activity of gonadotropin-releasing hormone (GnRH) and having an amino acid sequence obtained by deleting amino acids 2 and 3 from the N-terminal region of the peptide having the following amino acid sequence (I):
(I)
(SEQ ID NO: 7)
<Glu-Zaa-Zaa-His-Zaa-Ser-Zaa-Zaa-Zaa-Zaa-Pro-Gly-
NH 2
wherein <Glu represents pyroglutamic acid and Zaa represents any amino acid.
2 . The peptide according to claim 1 having the following amino acid sequence (3):
(3)
(SEQ ID NO: 6)
<Glu-His-Phe-Ser-Asn-Gly-Trp-His-Pro-Gly-NH 2
wherein <Glu represents pyroglutamic acid.
3 . An isolated precursor polypeptide of a peptide having the activity of gonadotropin-releasing hormone (GnRH) and having an amino acid of SEQ ID NO: 2.
4 . A DNA segment encoding the precursor polypeptide of claim 3 , which is a precursor of the peptide having the activity of gonadotropin-releasing hormone (GnRH).
5 . A DNA segment encoding a polypeptide having an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2.
6 . A DNA segment having a base sequence represented by SEQ ID NO: 3.
7 . A DNA segment capable of hybridizing with the DNA segment of any of claims 4 to 6 .
8 . A DNA segment having a homology of 60% or higher to the DNA segment of any one of claims 4 to 6 .
9 . A vector incorporating the DNA segment of any one of claims 4 to 6 .
10 . A host transformed by the vector of claim 9 .
11 . A method for producing a precursor polypeptide of a peptide having the activity of gonadotropin-releasing hormone (GnRH), comprising the steps of:
culturing or growing the host of claim 10 ; and collecting the precursor polypeptide of the peptide having the GnRH activity from the host or the culture solution of the host.
12 . A precursor polypeptide of a peptide having the activity of gonadotropin-releasing hormone (GnRH), obtained by the method of claim 11 .
13 . A method for producing a peptide having the activity of gonadotropin-releasing hormone (GnRH), comprising the steps of:
excising a precursor of the peptide having the GnRH activity from the precursor polypeptide of the peptide having the GnRH activity, the precursor polypeptide being obtained by the method of claim 11 ; and subsequently modifying the C-terminal and the N-terminal of the precursor.
14 . A DNA segment having a homology of 60% or higher to the DNA segment of claim 7 .
15 . A vector incorporating the DNA segment of claim 7 .
16 . A vector incorporating the DNA segment of claim 8 .Join the waitlist — get patent alerts
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