Biochemical labeling materials and manufacturing method thereof

Abstract

A biochemical labeling material and manufacturing method thereof. The manufacturing method provides a plurality of nanoparticles, bonding the nanoparticles to template molecules by molecular imprinting, polymerizing the nanoparticles to form a matrix with uniformly-distributed template molecules, finally removing the template molecules from the matrix to reveal a detection group of the matrix, leaving a cavity with specific area.

1 . (canceled) 
     
     
         2 . A method for manufacturing a biochemical labeling material comprising:
 providing a plurality of nanoparticles;   bonding the nanoparticles to a template molecule by molecular imprinting;   polymerizing the nanoparticles to form a matrix with uniformly-distributed template molecule; and   removing the template molecule from the matrix to reveal a detection group of the matrix and leave a cavity with specific area,   wherein the nanoparticles are metal.   
     
     
         3 . The method for manufacturing a biochemical labeling material as claimed in  claim 2 , wherein the nanoparticles are Au, Ag, Ni, or Co. 
     
     
         4 . A method for manufacturing a biochemical labeling material comprising:
 providing a plurality of nanoparticles;   bonding the nanoparticles to a template molecule by molecular imprinting;   polymerizing the nanoparticles to form a matrix with uniformly-distributed template molecule; and   removing the template molecule from the matrix to reveal a detection group of the matrix and leave a cavity with specific area,   wherein the nanoparticles are metal oxide.   
     
     
         5 . The method for manufacturing a biochemical labeling material as claimed in  claim 4 , wherein the nanoparticles are iron oxide. 
     
     
         6 - 17 . (canceled) 
     
     
         18 . A biochemical labeling material manufactured by a method comprising:
 providing a plurality of nanoparticles;   bonding the nanoparticles to a template molecule by molecular imprinting;   polymerizing the nanoparticles to form a matrix with uniformly-distributed template molecule; and   removing the template molecule from the matrix to reveal a detection group of the matrix and leave a cavity with specific area.   
     
     
         19 . A biochemical labeling material as claimed in  claim 18 , wherein the nanoparticles are metal. 
     
     
         20 . A biochemical labeling material as claimed in  claim 19 , wherein the nanoparticles are Au, Ag, Ni, or Co. 
     
     
         21 . A biochemical labeling material as claimed in  claim 18 , wherein the nanoparticles are metal oxide. 
     
     
         22 . A biochemical labeling material as claimed in  claim 21 , wherein the nanoparticles are iron oxide. 
     
     
         23 . A biochemical labeling material as claimed in  claim 18 , wherein the nanoparticles are semiconductors. 
     
     
         24 . A biochemical labeling material as claimed in  claim 23 , wherein the nanoparticles are II-VI or III-V group semiconductors. 
     
     
         25 . A biochemical labeling material as claimed in  claim 23 , wherein the nanoparticles have core-shell structure of at least two semiconductors. 
     
     
         26 . A biochemical labeling material as claimed in  claim 18 , wherein the surface of nanoparticles is further functionalized prior to the bonding with template molecule. 
     
     
         27 . A biochemical labeling material as claimed in  claim 26 , wherein the surface of nanoparticles is functionalized by fluorescent molecule. 
     
     
         28 . A biochemical labeling material as claimed in  claim 27 , wherein the fluorescent molecule is dansyl-chloride, anthracene, pyrene, coumarine, n-vinylcarbazole or derivatives thereof 
     
     
         29 . A biochemical labeling material as claimed in  claim 26 , wherein the surface of nanoparticles is functionalized by electron-transfer molecule. 
     
     
         30 . A biochemical labeling material as claimed in  claim 29 , wherein the electron-transfer molecule is amine, porphine, fullerene, organophosphine, carotene, or derivatives thereof 
     
     
         31 . A biochemical labeling material as claimed in  claim 26 , wherein the surface of nanoparticles is functionalized by (3-mercaptopropyl) trimethoxy silane or (3-aminopropyl) trimethoxy silane to form a functional group thereon, able to bond to the template molecule by hydrogen bond. 
     
     
         32 . A biochemical labeling material as claimed in  claim 26 , wherein the surface of nanoparticles is functionalized by (4-vinyl pyridine) or allyl mercaptothiol to form an unsaturated double-bond thereon. 
     
     
         33 . A biochemical labeling material as claimed in  claim 26 , wherein the surface of nanoparticles was functionalized by (3-mercaptopropyl) trimethoxy silane) or vinyl trimethoxy silane to form an unsaturated double-bond thereon. 
     
     
         34 . A biochemical labeling material as claimed in  claim 18 , wherein a functional monomer, a crosslinking agent, and an initiator are further added during polymerization of nanoparticles.