Isolation, Identification and Characterization of tmst2, a Novel Member of the TNF-Receptor Supergene Family

Abstract

Novel TNF receptor polypeptides are disclosed, along with polynucleotides encoding the polypeptides and uses thereof.

1 - 17 . (canceled) 
     
     
         18 . An antibody produced by immunizing an animal with a peptide comprising an amino acid sequence of SEQ ID NO: 8 or 10. 
     
     
         19 . An antibody or fragment thereof that specifically binds the polypeptide selected from the group consisting of:
 (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 8 or SEQ ID NO: 10;   (b) a polypeptide comprising amino acids 1-170 of SEQ ID NO: 8, wherein the polypeptide specifically binds TRAIL;   (c) a polypeptide comprising an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NOs: 7 or 9; and   (d) a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence set forth in SEQ ID NOs: 8 or 10, wherein said polypeptide is capable of binding TRAIL.   
     
     
         20 . The antibody of  claim 19  that is a monoclonal antibody. 
     
     
         21 . A hybridoma that produces a monoclonal antibody that binds to a peptide comprising an amino acid sequence of SEQ ID NO: 8 or 10. 
     
     
         22 . A method of detecting or quantitating the amount of tmst2-receptor polypeptide using the anti-tmst2-receptor antibody or fragment of  claim 18 . 
     
     
         23 . A selective binding agent or fragment thereof that specifically binds at least one polypeptide, wherein said polypeptide is selected from the group consisting of:
 (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 8 or SEQ ID NO: 10;   (b) a polypeptide comprising amino acids 1-170 of SEQ ID NO: 8, wherein the polypeptide specifically binds TRAIL;   (c) a polypeptide comprising an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NOs: 7 or 9; and   (d) a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence set forth in SEQ ID NOs: 8 or 10, wherein said polypeptide is capable of binding TRAIL.   
     
     
         24 . The selective binding agent of  claim 23  that is an antibody or fragment thereof. 
     
     
         25 . The selective binding agent of  claim 23  that is a humanized antibody. 
     
     
         26 . The selective binding agent of  claim 23  that is a human antibody or fragment thereof. 
     
     
         27 . The selective binding agent of  claim 23  that is a polyclonal antibody or fragment thereof. 
     
     
         28 . The selective binding agent  claim 23  that is a monoclonal antibody or fragment thereof. 
     
     
         29 . The selective binding agent of  claim 23  that is a chimeric antibody or fragment thereof. 
     
     
         30 . The selective binding agent of  claim 23  that is a CDR-grafted antibody or fragment thereof. 
     
     
         31 . The selective binding agent of  claim 23  that is an antiidiotypic antibody or fragment thereof. 
     
     
         32 . The selective binding agent of  claim 23  which is a variable region fragment. 
     
     
         33 . The variable region fragment of  claim 32  which is a Fab or a Fab′ fragment. 
     
     
         34 . A selective binding agent or fragment thereof comprising at least one complementarity determining region with specificity for a polypeptide having the amino acid sequence of SEQ ID NO: 8 or 10. 
     
     
         35 . The selective binding agent of  claim 23  which is bound to a detectable label. 
     
     
         36 . The selective binding agent of  claim 23  which antagonizes tmst2-receptor polypeptide biological activity. 
     
     
         37 . A method for treating, preventing, or ameliorating a disease, condition, or disorder comprising administering to a patient an effective amount of a selective binding agent according to  claim 23 . 
     
     
         38 . A selective binding agent produced by immunizing an animal with a polypeptide comprising an amino acid sequence of SEQ ID NO: 8 or 10. 
     
     
         39 . A hybridoma that produces a selective binding agent capable of binding a polypeptide selected from the group consisting of:
 (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 8 or SEQ ID NO: 10;   (b) a polypeptide comprising amino acids 1-170 of SEQ ID NO: 8, wherein the polypeptide specifically binds TRAIL;   (c) a polypeptide comprising an amino acid sequence encoded by the nucleotide sequence set forth in SEQ ID NOs: 7 or 9; and   (d) a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence set forth in SEQ ID NOs: 8 or 10, wherein said polypeptide is capable of binding TRAIL.   
     
     
         40 - 63 . (canceled) 
     
     
         64 . A method of detecting or quantitating the amount of tmst2-receptor polypeptide using the anti-tmst2-receptor antibody or fragment of  claim 19 .