Transgenic mammal carrying GANP gene transferred thereinto and utilization thereof

Abstract

It is an object of the present invention to provide a high affinity antibody effective as a diagnostic or therapeutic for various diseases; a transgenic mammal for producing the high affinity antibody; and a medicine comprising the high affinity antibody or a cell producing the high affinity antibody. According to the present invention, a transgenic mammal carrying a GANP gene transferred thereinto, its progeny, or a part thereof, and a method of producing a high affinity antibody using the same are provided.

1 . A high affinity antibody or a fragment thereof produced by a process of:
 administering an antigen to a transgenic non-human mammal or its progeny and recovering an antibody from the resultant mammal or progeny,   wherein said transgenic non-human mammal comprises a GANP gene.   
     
     
         2 . The antibody according to  claim 1 , wherein said antibody has an affinity of 1×10 −7  M or less as expressed as a dissociation constant, or a fragment thereof. 
     
     
         3 . The antibody according to  claim 1 , wherein said antibody is a polyclonal antibody or a monoclonal antibody, or a fragment thereof. 
     
     
         4 . The antibody according to  claim 1 , wherein said antibody is a humanized antibody or a human antibody, or a fragment thereof, and wherein said antibody comprises a V region of said antibody or a fragment thereof. 
     
     
         5 . A pharmaceutical composition comprising the antibody according to  claim 1 , or a fragment thereof. 
     
     
         6 . The pharmaceutical composition according to  claim 5 , wherein said antibody or a fragment thereof is a humanized antibody or a fragment thereof. 
     
     
         7 . The pharmaceutical composition according to  claim 5 , wherein said antibody or a fragment thereof is a human antibody or a fragment thereof.