Cleaning compositions comprising transglucosidase

Abstract

Provided herein is a composition comprising: a) a transglucosidase enzyme; and b) a natural gum polysaccharide, wherein said natural gum polysaccharide is a substrate for said transglucosidase enzyme. A method of using a transglucosidase enzyme to a degrade natural gum polysaccharide is also provided. The composition and method may be employed in cleaning applications.

1 . A composition comprising:
 a) transglucosidase enzyme; and   b) natural gum polysaccharide;
 wherein said natural gum polysaccharide is a substrate for said transglucosidase enzyme. 
   
     
     
         2 . The composition of  claim 1 , wherein said natural gum polysaccharide is xanthan gum. 
     
     
         3 . The composition of  claim 1 , wherein said transglucosidase enzyme has an activity defined as EC 2.4.1.24, according to IUBMB nomenclature. 
     
     
         4 . The composition of  claim 1 , wherein said transglucosidase enzyme has an amino acid sequence that is at least 90% identical to an  Aspergillus  transglucosidase enzyme. 
     
     
         5 . The composition of  claim 1 , wherein said composition further comprises a surfactant. 
     
     
         6 . The composition of  claim 1 , wherein said natural gum polysaccharide is present as a stain on an object. 
     
     
         7 . The composition of  claim 6 , wherein said object is a fabric. 
     
     
         8 . A method comprising combining a transglucosidase enzyme with a natural gum polysaccharide to degrade said natural gum polysaccharide. 
     
     
         9 . The method of  claim 8 , wherein said transglucosidase enzyme has an activity defined as EC 2.4.1.24, according to IUBMB nomenclature. 
     
     
         10 . The method of  claim 8 , wherein said natural gum polysaccharide is xanthan gum. 
     
     
         11 . A cleaning method comprising:
 a) contacting an object soiled with a natural gum polysaccharide with a cleaning composition comprising a transglucosidase enzyme; and   b) maintaining said object and cleaning composition under conditions sufficient to effect degradation of said natural gum polysaccharide and thereby clean said object.   
     
     
         12 . The cleaning method of  claim 11 , wherein said object is soiled with a food that contains said natural gum polysaccharide. 
     
     
         13 . The cleaning method of  claim 11 , wherein said natural gum polysaccharide is a xanthan gum. 
     
     
         14 . The cleaning method of  claim 11 , wherein said object is selected from fabrics and hard surfaces. 
     
     
         15 . The cleaning method of  claim 11 , wherein said transglucosidase enzyme has an amino acid sequence that is at least 90% identical to an  Aspergillus  transglucosidase enzyme. 
     
     
         16 . The cleaning method of  claim 11 , wherein said cleaning composition further comprises a surfactant. 
     
     
         17 . The cleaning method of  claim 11 , wherein said cleaning composition further comprises at least one enzyme selected from proteases, amylases, cellulases, lipases, cutinases, mannanases, pectinases, pectate lyases and oxido-reductases, for the degradation of other stain components. 
     
     
         18 . The cleaning method of  claim 11 , wherein said cleaning composition has a pH of pH 5.0 to pH 11.5. 
     
     
         19 . The cleaning method of  claim 11 , wherein said cleaning composition comprises said enzyme at a concentration in the range of 0.01 ppm to 100 ppm.