Methods of Modulating Glucosinolate Production in Plants

Abstract

The present invention relates to methods for modulating glucosinolate production in plants, specifically by modulating CYP83A1 expression. The present invention also relates to transgenic plants that over-express and underexpress CYP83A1.

1 .- 38 . (canceled) 
     
     
         39 . A transgenic plant that overexpresses CYP83A1 relative to a corresponding wild-type plant, wherein said transgenic plant has increased cell length, increased leaf size, increased height, or increased seed production relative to the wild type plant. 
     
     
         40 . A method of producing a transgenic plant with CYP83A1 overexpression relative to a wild-type plant, said method comprising: (a) introducing an expression construct that comprises a polynucleotide encoding a CYP83A1 polypeptide operably linked to a promoter which is capable of overexpressing the polypeptide into a plant cell to produce a transformed plant cell; and (b) producing a transgenic plant from the transformed plant cell, wherein the transgenic plant has increased cell length, increased leaf size, increased height, or increased seed production relative to a wild type plant. 
     
     
         41 . The method of claim  2 , wherein the polynucleotide is operably linked to a promoter selected from the group consisting of a tissue-specific promoter, an inducible promoter and a constitutive promoter.