Environmental stress-responsive promoter and a gene encoding environmental stress-responsive transcriptional factor

Abstract

The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

1 .- 4 . (canceled) 
     
     
         5 . A method of regulating expression of a gene which comprises:
 (a) preparing a recombinant plant cell line, plant tissue or plant comprising an expression vector having an abiotic environmental stress-responsive promoter comprising SEQ ID NO: 63 which is operably linked to the coding sequence the gene; and   (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.   
     
     
         6 . The method according to  claim 5 , wherein the abiotic environmental stress is cold stress, drought stress or salt stress. 
     
     
         7 . The method according to  claim 5 , wherein the gene encodes a polypeptide that can confer increased environmental stress resistance compared to a plant cell line, plant tissue or plant lacking the expression vector. 
     
     
         8 . The method according to  claim 5 , wherein the gene is a plant gene.