Genes Associated With Type ll Diabetes Mellitus

Abstract

Genes associated with Type II Diabetes Mellitus (T2DM) are identified and screening methods to identify chemical compounds that act on those targets for the treatment of T2DM or its associated pathologies are discussed. The use of EP3 antagonist was found to affect blood glucose levels in mice fed a high-fat diet and made insulin deficient by streptozocin injection.

1 . A method of screening small molecule compounds for use in treating Type II Diabetes Mellitus (T2DM), comprising screening a test compound against a target selected from the group consisting of Prostaglandin E Receptor 3 (EP3), inducible Nitric Oxide Synthase (iNOS) BRAF, Caspase 8, Integrin Alpha 9 (ITGA9), ADAMTS7 (A Disintegrin-like And Metalloprotease domain with Thrombospondin motifs 7) and Amyloid Precursor Protein, where activity against said target indicates the test compound has potential use in treating Type II Diabetes Mellitus. 
     
     
         2 . A method according to  claim 1  wherein the target is EP3. 
     
     
         3 . A method of lowering serum blood glucose levels in a mammalian subject in need thereof, comprising administering to said subject an EP3 antagonist in an amount effective to decrease serum blood glucose levels compared to the level of serum blood glucose that would be seen in the absence of said administration. 
     
     
         4 . A method according to  claim 3  wherein the subject is a human with Type II diabetes mellitus (T2D). 
     
     
         5 . A method of increasing glucose uptake from the blood in a mammalian subject in need thereof, comprising administering to said subject an EP3 antagonist in an amount effective to increase uptake of blood glucose compared to the level of uptake that would be seen in the absence of said administration. 
     
     
         6 . A method according to  claim 5  wherein the subject is a human with Type II diabetes mellitus (T2D).