TML polynucleotides

Abstract

The present invention is directed to polynucleotides, peptides, variants, and uses thereof for a novel peptide fragment designated TML peptides. Binding of the peptide fragment has been shown in kidney and small intestine. The present invention further includes agonists, antagonists, variants, antibodies, host cells expressing the cDNA encoding the novel TML peptides and methods for increasing gastric motility and secretion of digestive proteins and hormones using the novel TML peptides.

1 . An isolated polynucleotide molecule encoding an isolated peptide molecule as shown in SEQ ID NO:12, said peptide molecule consisting of residues X through Y, wherein X is an integer from 1 to 4, inclusive, and wherein Y is 14 or 18, and wherein at least (Y minus X) minus 2 residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         2 . An isolated polynucleotide molecule according to  claim 1 , wherein at least (Y minus X) minus 1 residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         3 . An isolated polynucleotide molecule according to  claim 2 , wherein at least (Y minus X) residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         4 . The isolated peptide molecule of  claim 1 . 
     
     
         5 . A method of modulating contractility in duodenum or jejunum tissue comprising applying the isolated peptide of  claim 4  to said tissue. 
     
     
         6 . A method of modulating pancreatic secretion of hormones and digestive enzymes comprising administering the isolated peptide of  claim 4  to a mammal. 
     
     
         7 . An isolated polynucleotide molecule encoding an isolated peptide molecule as shown in SEQ ID NO:12, said peptide molecule consisting of residues X through 11, wherein X is 1 or 2, and wherein at least (11 minus X) minus 2 residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         8 . An isolated polynucleotide molecule according to  claim 7 , wherein at least (11 minus X) minus 1 residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         9 . An isolated polynucleotide molecule according to  claim 9 , wherein at least 11 minus X residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         10 . The isolated peptide molecule of  claim 7 . 
     
     
         11 . A method of modulating contractility in duodenum or jejunum tissue comprising applying the isolated peptide of  claim 10  to said tissue. 
     
     
         12 . A method of modulating pancreatic secretion of hormones and digestive enzymes comprising administering the isolated peptide of  claim 10  to a mammal. 
     
     
         13 . An isolated polynucleotide molecule encoding an isolated peptide molecule as shown in SEQ ID NO:12, said peptide molecule consisting of residues 1 through 10, and wherein at least seven residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         14 . An isolated polynucleotide according to  claim 13 , wherein at least eight residues are as in the corresponding region of SEQ ID NO:11. 
     
     
         15 . An isolated polynucleotide according to  claim 13 , wherein at least nine are as in the corresponding region of SEQ ID NO:11. 
     
     
         16 . The isolated peptide molecule of  claim 13 . 
     
     
         17 . A method of modulating contractility in duodenum or jejunum tissue comprising applying the isolated peptide of  claim 16  to said tissue. 
     
     
         18 . A method of modulating pancreatic secretion of hormones and digestive enzymes comprising administering the isolated peptide of  claim 16  to a mammal. 
     
     
         19 . An isolated polynucleotide molecule encoding an isolated peptide, wherein the peptide is selected from the group consisting of:
 a) residues 2 to 18 of SEQ ID NO:11;   b) residues 2 to 14 of SEQ ID NO:11;   c) residues 3 to 18 of SEQ ID NO:11;   d) residues 3 to 14 of SEQ ID NO:11;   e) residues 4 to 18 of SEQ ID NO:11;   f) residues 4 to 14 of SEQ ID NO:11;   g) residues 1 to 11 of SEQ ID NO:11;   h) residues 1 to 10 of SEQ ID NO:11; and   i) residues 2 to 11 of SEQ ID NO:11.   
     
     
         20 . The isolated peptide molecule of  claim 19 . 
     
     
         21 . A method of modulating contractility in duodenum or jejunum tissue comprising applying the isolated peptide of  claim 20  to said tissue. 
     
     
         22 . A method of modulating pancreatic secretion of hormones and digestive enzymes comprising administering the isolated peptide of  claim 20  to a mammal.