Novel mixture and compounds from mycelia of Antrodia camphorata and use thereof

Abstract

The present invention relates to novel mixtures and maleic and succinic acid derivatives from mycelium of Antrodia camphorata and the medical use thereof. The present invention relates to the composition or mycelium comprising the compounds of the invention.

1 .- 14 . (canceled) 
     
     
         15 . A compound having the formula 
       
         
           
           
               
               
           
         
         wherein 
         X is N or O; 
         R 1  is C 1-10  alkyloxy, C 2-10  alkenyloxy, or C 2-10  alkynyloxy; 
         R 2  is H, C 1-10  alkyl, C 2-10  alkenyl or C 2-10  alkynyl; and 
         R 3  is absent, H or hydroxy; 
         provided that if X is O, R 3  is absent. 
       
     
     
         16 . The compound of  claim 15 , wherein R 1  is C 2-6  alkenyloxy or C 2-6  alkynyloxy. 
     
     
         17 . The compound of  claim 16 , wherein C 2-6  alkenyloxy is substituted with C 1-6  alkyl. 
     
     
         18 . The compound of  claim 15 , wherein R 2  is isobutyl. 
     
     
         19 . The compound of  claim 15 , which is 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]furan-2,5-dione, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione, 3R*, 4S*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, or 3R*, 4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione. 
     
     
         20 . A composition comprising a compound having the formula 
       
         
           
           
               
               
           
         
         wherein 
         X is N or O; 
         R 1  is C 1-10  alkyloxy, C 2-10  alkenyloxy, or C 2-10  alkynyloxy; 
         R 2  is H, C 1-10  alkyl, C 2-10  alkenyl or C 2-10  alkynyl; and 
         R 3  is absent, H or hydroxy; 
         provided that if X is O, R 3  is absent. 
       
     
     
         21 . The composition of  claim 20 , wherein the compound is 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]furan-2,5-dione, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-2,5-dione, 3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]-1H-pyrrol-1-ol-2,5-dione, 3R*, 4S*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione, or 3R*, 4R*-1-hydroxy-3-isobutyl-4-[4-(3-methyl-2-butenyloxy)phenyl]pyrrolidine-2,5-dione. 
     
     
         22 . The composition of  claim 20 , which decreases systolic blood pressure or increases high density lipoprotein. 
     
     
         23 . The composition of  claim 20 , which has central cholinergic agonism, hepatoprotection, anti-inflammation or anti-tumor activity. 
     
     
         24 . The composition of  claim 20 , wherein tumor is from the cells or tissues selected from the group consisting of liver, intestine, bone, blood, lymph and breast.