Single-chain Antibodies Against Human Insulin-Like Growth Factor I Receptor: Expression, Purification, and Effect on Tumor Growth

Abstract

A method of inhibiting the growth of hormone dependent tumor cells in a mammal comprises administering to said mammal an insulin-like growth factor receptor (IGF-IR) recombinant antibody, wherein said antibody can be a single-chain recombinant antibody, which can be humanized, capable of blocking agonist interaction with the IGF-IR.

1 . A purified recombinant antibody comprising antigen binding portions having the binding specificity of the antigen binding portions of murine antibody 1H7. 
     
     
         2 . A purified recombinant antibody comprising antigen binding portions of a V L  domain and a V H  domain comprising amino acid sequences as set forth in SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, and in SEQ ID NO:9, SEQ ID NO:10, and SEQ ID NO:11, respectively. 
     
     
         3 . The recombinant antibody of  claim 2 , wherein the V L  and V H  domains have amino acid sequences as set forth in SEQ ID NO:3 and SEQ ID NO:4, respectively.