Cardiovascular tissue culture substrate

Abstract

The substrate for culturing a cardiovascular tissue of the present invention is a substrate for culturing a cardiovascular tissue, which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material, the foam comprising lactide (D, L, DL isomer)-ε-caprolactone copolymer containing lactide (D, L, DL isomer) in a content of 50 to 54 mole % and ε-caprolactone in a content of 50 to 46 mole %, and the reinforcing material being covered with the foam.

1 . A substrate for culturing a cardiovascular tissue,
 which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,   the foam comprising lactide (D, L, DL isomer)-ε-caprolactone copolymer containing lactide (D, L, DL isomer) in a content of 50 to 54 mole % and ε-caprolactone in a content of 50 to 46 mole %,   and the reinforcing material being covered with the foam.   
     
     
         2 . A substrate for culturing a cardiovascular tissue,
 which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,   the foam having a thickness of 0.2 to 3.0 mm,   the reinforcing material being situated at a center or on an outer plane, and an inner plane comprising the foam.   
     
     
         3 . A substrate for culturing a cardiovascular tissue,
 which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,   the reinforcing material comprising a bioabsorbable fiber coated with a bioabsorbable material,   the reinforcing material being situated at a center or on an outer plane, and an inner plane comprising the foam.   
     
     
         4 . The substrate for culturing a cardiovascular tissue according to  claim 3 ,
 wherein the reinforcing material comprises a polyglycolic acid fiber coated with lactide (D, L, DL isomer)-ε-caprolactone copolymer.   
     
     
         5 . A substrate for culturing a cardiovascular tissue,
 which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material,   the reinforcing material comprising a twisted yarn comprising a twisted bioabsorbable multifilament yarn,   the reinforcing material being situated at a center or on an outer plane, and an inner plane comprising the foam.   
     
     
         6 . A substrate for culturing a cardiovascular tissue,
 which is tubular, and comprises a foam comprising a bioabsorbable material reinforced with a reinforcing material comprising a bioabsorbable material, and a reinforcing yarn comprising a bioabsorbable material,   the reinforcing yarn and the reinforcing material being situated at a center or on an outer plane of the foam, and an inner plane comprising the foam.   
     
     
         7 . The substrate for culturing a cardiovascular tissue according to  claim 6 ,
 wherein the reinforcing yarn comprises at least one kind of compound selected from the group consisting of poly-L-lactide, lactide (D, L, DL isomer)-ε-caprolactone copolymer and glycolic acid-ε-caprolactone copolymer.   
     
     
         8 . The substrate for culturing a cardiovascular tissue according to  claim 6 ,
 wherein the reinforcing yarn is wound in a spiral form, a ring form, or a X-shaped form.   
     
     
         9 . A method of producing a cardiovascular tissue for transplantation, wherein a substrate surface is covered with a cell,
 which comprises seeding a cell in vitro on the substrate for culturing a cardiovascular tissue according to  claim 1 ,  2 ,  3 ,  4 ,  5 ,  6 ,  7  or  8 , and further culturing the cell in vitro.   
     
     
         10 . A method of regenerating a cardiovascular tissue,
 which comprises seeding a cell in vitro on the substrate for culturing a cardiovascular tissue according to  claim 1 ,  2 ,  3 ,  4 ,  5 ,  6 ,  7  or  8 , and further culturing the cell to regenerate a cardiovascular tissue in vitro.   
     
     
         11 . A cardiovascular tissue for transplantation,
 which is obtained by seeding an endothelial cell, a marrow cell, a smooth muscle cell or a fibroblast in vitro on the substrate for culturing a cardiovascular tissue according to  claim 1 ,  2 ,  3 ,  4 ,  5 ,  6 ,  7  or  8 , and further culturing them in vitro.