US2008171351A1PendingUtilityA1

Probes for anionic cell surface detection

53
Assignee: UNIV NOTRE DAME DU LACPriority: May 22, 2006Filed: May 16, 2007Published: Jul 17, 2008
Est. expiryMay 22, 2026(expired)· nominal 20-yr term from priority
Inventors:Bradley D. Smith
G01N 2510/00G01N 33/92C12Q 1/04
53
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Claims

Abstract

Embodiments of the present invention are generally directed to compositions comprising a class of molecular probes for detecting the presence of anionic cell surfaces. Embodiments include compositions that are enriched for these compositions and preparations, particularly preparations suitable for use as laboratory/clinical reagents and diagnostic indicators, either alone or as part of a kit. An embodiment of the invention provides for a highly selective agent useful in the discernment and identification of dead or dying cells, such as apoptotic cells, in a relatively calcium-free environment. An embodiment of the invention provides a selective agent for the identification of bacteria in a mixed population of bacterial cells and nonbacterial cells.

Claims

exact text as granted — not AI-modified
1 . A construct for detecting the presence of an anionic cell surface element, said construct comprising the following structure: 
       
         
           
           
               
               
           
         
         wherein L is a linker, and R is a reporter element. 
       
     
     
         2 . The construct of  claim 1 , wherein said linker is an ethyleneoxy linker. 
     
     
         3 . The construct of  claim 1 , wherein said linker is a (tris)ethyleneoxy linker. 
     
     
         4 . The construct of  claim 1 , wherein said linker is a butyl linker. 
     
     
         5 . The construct of  claim 1 , wherein said reporter element is a fluorescent molecule or a dye. 
     
     
         6 . The construct of  claim 1 , wherein said reporter element is an MRI-contrast agent or a radioactive agent. 
     
     
         7 . The construct of  claim 1 , wherein said reporter element has the following structure: 
       
         
           
           
               
               
           
         
       
     
     
         8 . The construct of  claim 1 , wherein said reporter element has the following structure: 
       
         
           
           
               
               
           
         
       
     
     
         9 . The construct of  claim 1 , wherein said reporter element comprises a quantum dot. 
     
     
         10 . The construct of  claim 9 , wherein said quantum dot comprises a CdSe/CdS core/shell nanocrystal. 
     
     
         11 . The construct of  claim 9 , wherein said quantum dot is linked to at least one additional construct having the following structure: 
       
         
           
           
               
               
           
         
       
       in which L is a linker, and R is the quantum dot. 
     
     
         12 . The construct of  claim 1 , wherein said reporter element has the following structure: 
       
         
           
           
               
               
           
         
       
     
     
         13 . The construct of  claim 1 , wherein said anionic cell surface element comprises monoanionic phospholipids. 
     
     
         14 . The construct of  claim 13 , wherein said monoanionic phospholipids comprise phosphatidylserine. 
     
     
         15 . The construct of  claim 1 , wherein said anionic cell surface element is a bacterial cell surface. 
     
     
         16 . The construct of  claim 1 , wherein said construct has a molecular weight of less than 5000 MW. 
     
     
         17 . A method for determining the presence of an anionic cell surface element in a sample, comprising:
 introducing a construct having the following structure to the sample   
       
         
           
           
               
               
           
         
         wherein L is a linker, and R is a reporter element, and wherein the construct binds to the anionic cell surface element of the sample; and 
         detecting the presence of the anionic cell surface element of the sample by fluorescence emission from the construct. 
       
     
     
         18 . The method of  claim 17 , wherein said anionic cell surface element comprises monoanionic phospholipids. 
     
     
         19 . The method of  claim 18 , wherein said monoanionic phospholipids comprise phosphatidylserine. 
     
     
         20 . The method of  claim 17 , wherein said method is carried out in a low calcium environment. 
     
     
         21 . The method of  claim 17 , wherein said method is carried out in a substantially calcium free environment. 
     
     
         22 . The method of  claim 17 , wherein binding of said construct to said anionic cell surface element is independent of calcium concentration. 
     
     
         23 . The method of  claim 17 , wherein said construct has a molecular weight of less than 5000 MW. 
     
     
         24 . The method of  claim 17 , wherein said reporter element is a fluorescent molecule or a dye. 
     
     
         25 . The method of  claim 17 , wherein said reporter element is an MRI-contrast agent or a radioactive agent. 
     
     
         26 . The method of  claim 17 , wherein said reporter element has the following structure: 
       
         
           
           
               
               
           
         
       
     
     
         27 . The method of  claim 17 , wherein said reporter element has the following structure: 
       
         
           
           
               
               
           
         
       
     
     
         28 . The method of  claim 17 , wherein said reporter element comprises a quantum dot. 
     
     
         29 . The method of  claim 28 , wherein said quantum dot comprises a CdSe/CdS core/shell nanocrystal. 
     
     
         30 . The method of  claim 28 , wherein said quantum dot is linked to at least one additional construct having the following structure: 
       
         
           
           
               
               
           
         
       
       in which L is a linker, and R is the quantum dot. 
     
     
         31 . The method of  claim 17 , wherein said reporter element has the following structure: 
       
         
           
           
               
               
           
         
       
     
     
         32 . The method of  claim 17 , wherein said anionic cell surface element is a bacterial cell surface.

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