Anti-cd33 antibodies and method for treatment of acute myeloid leukemia using the same

Abstract

The present invention relates to antibodies that bind CD33. More particularly, the invention relates to anti-CD33 antibodies, fragments and homologues of these antibodies, humanized and resurfaced versions of these antibodies, functional equivalents and improved versions of these antibodies, immunoconjugates and compositions comprising these antibodies, and the uses of same in diagnostic, research and therapeutic applications. The invention also relates to a polynucleotide encoding these antibodies, vectors comprising the polynucleotides, host cells transformed with polynucleotides and methods of producing these antibodies.

1 . At least one isolated polynucleotide encoding an antibody or epitope-binding fragment thereof, that specifically binds to CD33, comprising a heavy chain variable region and a light chain variable region, wherein said heavy chain variable region comprises the amino acid sequences of SEQ ID NOs: 1-3, wherein said antibody or said fragment comprises at least one complementarity-determining region having an amino acid sequence selected from the group consisting of SEQ ID NOs:1-6: 
       
         
           
                 
                 
                 
                 
               
                     
                   SYYIH, 
                   (SEQ ID NO:1) 
                     
                 
                     
                     
                 
                     
                   VIYPGNDDISYNQKFXG, 
                   (SEQ ID NO:2)  
                 
                     
                   wherein X is K or Q, 
                 
                     
                     
                 
                     
                   EVRLRYFDV, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   KSSQSVFFSSSQKNYLA, 
                   (SEQ ID NO:4) 
                 
                     
                     
                 
                     
                   WASTRES, 
                   (SEQ ID NO:5) 
                 
                     
                     
                 
                     
                   HQYLSSRT, 
                   (SEQ ID NO:6) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
         and wherein said antibody or said fragment binds CD33.