Cloning and sequencing of the allergen DAC G 5 of dactylis glomerata pollen, its preparation and use thereof

Abstract

A purified nucleic acid molecule comprising a nucleotide sequence coding for allergen Dac g 5 having amino acid sequence SEQ ID NO. 2, a derivative or a fragment thereof.

1 . A recombinant protein comprising the allergen Dac g 5 or a derivative thereof obtained by culturing a prokaryote or eukaryote organism transformed by a nucleic acid molecule comprising a nucleotide sequence coding for allergen Dac g 5 having amino acid sequence SEQ ID NO. 2, a derivative or a fragment thereof, under conditions and for a sufficient length of time to enable expression of said protein, and isolating proteins produced from the transformed organisms. 
     
     
         2 . A recombinant protein comprising the allergen Dac g 5 or a derivative thereof obtained by culturing a prokaryote or eukaryote organism transformed by an expression vector comprising a purified nucleic acid molecule comprising a nucleotide sequence coding for allergen Dac g 5 having amino acid sequence SEQ ID NO. 2, a derivative or a fragment thereof, under conditions and for a sufficient length of time to enable expression of the protein, and isolating proteins produced from the transformed organisms. 
     
     
         3 . A pharmaceutical composition for treating or diagnosing an allergy, comprising a therapeutically effective amount of a protein according to  claim 1  or an antibody directed against the protein. 
     
     
         4 . A reagent for diagnosing an allergy comprising a protein according to  claim 1  or an antibody directed against the protein. 
     
     
         5 . A pharmaceutical composition for treating or diagnosing an allergy, comprising a therapeutically effective amount of a protein according to  claim 2  or an antibody directed against the protein. 
     
     
         6 . A reagent for diagnosing an allergy comprising a protein according to  claim 2  or an antibody directed against the protein. 
     
     
         7 . A process for detecting sensitivity manifested by an individual to pollen of  Dactylis glomerata , comprising:
 contacting a sample obtained from an individual with a protein according to  claim 1  or an antibody directed against the protein under conditions enabling formation of an antigen/antibody complex, and   detecting presence of the complex.