Stimulation of in vivo production of proteins

Abstract

The invention relates to a formulation in the form of a pharmaceutical composition, a food product, a feed product or a dietary supplement, comprising leucine and protein in specific amounts. Consumption of a formulation according to the invention has a very positive effect on the generation of muscle tissue and is therefore particularly useful for organisms wherein an anabolic response is desired.

1 . A composition comprising leucine and proteinaceous matter, wherein leucine is present in an amount of at least about 10 wt. % based on a total dry weight of the composition, and wherein leucine is present in a weight ratio of between about 0.2 and 0.4 leucine to other amino acids present in the proteinaceous matter. 
     
     
         2 . The composition of  claim 1 , wherein leucine is present in an amount of about 12 to 80 wt. % based on the total dry weight of the composition. 
     
     
         3 . The composition of  claim 1 , wherein leucine is present in an amount of about 20 to 60 wt. % based on the total dry weight of the composition. 
     
     
         4 . The composition of  claim 1 , wherein leucine comprises L-leucine. 
     
     
         5 . The composition of  claim 1 , wherein leucine comprises free leucine. 
     
     
         6 . The composition of  claim 1  in a daily dosage amount, wherein leucine is present in a weight ratio of greater than about 0.48 leucine to a total amount of branched amino acids. 
     
     
         7 . The composition of  claim 6 , wherein leucine is present in a weight ratio of between about 0.88 and 20 leucine to a sum of valine and isoleucine. 
     
     
         8 . The composition of  claim 6 , wherein leucine is present in a weight ratio of between about 1.1 and 10 leucine to a sum of valine and isoleucine. 
     
     
         9 . The composition of  claim 1  in a daily dosage amount, wherein leucine is present in a weight ratio of between about 0.29 and 0.35 leucine to other amino acids. 
     
     
         10 . The composition of  claim 1  in a dosage unit, wherein at least about 0.037 grams leucine is present per kilogram bodyweight per dosage unit. 
     
     
         11 . The composition of  claim 1  in a dosage unit, wherein at least 0.04 grams leucine is present per kilogram bodyweight per dosage unit. 
     
     
         12 . The composition of  claim 1 , wherein the proteinaceous matter comprises at least about 90 wt. % intact proteins and/or peptides. 
     
     
         13 . The composition of  claim 1 , wherein the proteinaceous matter is present in an amount such that the composition comprises about 40 to 80 wt. % amino acids, based on the total dry weight of the composition. 
     
     
         14 . The composition of  claim 1 , wherein the proteinaceous matter is present in such an amount such that the composition comprises about 60 to 75 wt. % amino acids, based on the total dry weight of the composition. 
     
     
         15 . The composition of  claim 1  further comprising at least about 5 grams of essential amino acids. 
     
     
         16 . The composition of  claim 15  comprising synthetic L-lysine and/or synthetic L-methionine. 
     
     
         17 . The composition of  claim 1  further comprising one or more vitamins selected from the group consisting of folic acid, vitamin B6, vitamin B1, vitamin B2, biotin, lipoic acid, and vitamin B12. 
     
     
         18 . The composition of  claim 1  further comprising mono- and disaccharides, wherein the mono- and disaccharides are present in an amount of less than about 20 wt. %, based on the total dry weight of the composition. 
     
     
         19 . The composition of  claim 1  further comprising mono- and disaccharides, wherein the mono- and disaccharides are present in an amount of less than about 10 wt. %, based on the total dry weight of the composition. 
     
     
         20 . The composition of  claim 1  further comprising mono- and disaccharides, wherein the mono- and disaccharides are present in an amount of less than about 5 wt. %, based on the total dry weight of the composition. 
     
     
         21 . The composition of  claim 1 , wherein the composition is in a form selected from the group consisting of a pharmaceutical composition, a food product, and a dietary supplement. 
     
     
         22 . A food bar comprising:
 the composition of  claim 1  present in an amount about 20-40 wt. % of the food bar;   a carbohydrate present in an amount about 20-40 wt. % of the food bar; and   a lipid present in an amount about 2-10 wt. % of the food bar.   
     
     
         23 . The food bar of  claim 22  further comprising conventional food bar ingredients. 
     
     
         24 . A method for provoking an anabolic response in a mammal comprising administering to a mammal the formulation of  claim 1  in an amount sufficient to provoke an anabolic response. 
     
     
         25 . The method of  claim 24  wherein the formulation induces in vivo production of protein. 
     
     
         26 . The method of  claim 25  wherein the production of protein leads to generation of muscle tissue. 
     
     
         27 . The method of  claim 26  wherein the formulation is administered less than 2 hours prior to physical exercise. 
     
     
         28 . The method of  claim 25  wherein the production of protein leads to generation of skin tissue. 
     
     
         29 . The method of  claim 24  wherein catabolism is decreased in the mammal.