Adsorbent For Lymphocyte Proliferation Inhibitor and Treating Method

Abstract

The invention has for its object to provide a porous material capable of relieving the lymphocyte proliferation inhibition in lymphocyte culture and improving the proliferative nature of lymphocytes as well as a method for proliferating lymphocytes and a method for producing lymphocytes each of which utilizes such porous material. The invention relates to a porous material for body fluid treatment for promoting lymphocyte proliferation in lymphocyte culture which contains a high-molecular compound having an angle of contact with water within the range of 40° to 98 °; and a porous material for body fluid treatment which comprises activated carbon. It also relates to a treatment device wherein the porous material is used; a method for proliferating lymphocytes; a method for producing mammalian lymphocytes; a method for producing a pharmaceutical composition; an additive body fluid to be added to a culture medium on the occasion of lymphocyte culture; a method for treating a disease against which a therapeutic effect is produced by returning extracorporeally activated mammalian lymphocytes into the body; and the use of activated carbon or a high-molecular compound having an angle of contact with water within the range of 40° to 98° in manufacturing porous materials for body fluid treatment for promoting the lymphocyte proliferation in lymphocyte culture.

1 . A porous material for body fluid treatment for promoting lymphocyte proliferation in lymphocyte culture which contains a high-molecular compound having an angle of contact with water within the range of 40° to 98°. 
     
     
         2 . The porous material according to  claim 1   which has a molecular-weight exclusion limit of not higher than 1.5×10 5 .   
     
     
         3 . The porous material according to  claim 1 ,
 wherein the high-molecular compound is an aromatic high-molecular compound.   
     
     
         4 . The porous material according to  claim 3 ,
 wherein the aromatic high-molecular compound is polystyrene or a styrene-divinylbenzene copolymer.   
     
     
         5 . The porous material according to  claim 1 ,
 wherein the high-molecular compound contains no amine residue bound thereto.   
     
     
         6 . The porous material according to  claim 1 ,
 wherein the high-molecular compound has no other compound immobilized thereon.   
     
     
         7 . A porous material for body fluid treatment for promoting lymphocyte proliferation in lymphocyte culture which comprises activated carbon. 
     
     
         8 . A treatment device for promoting lymphocyte proliferation in lymphocyte culture which comprises the porous material according to  claim 1  and a container therefor. 
     
     
         9 . The device according to  claim 8 ,
 wherein said container has a liquid inlet and a liquid outlet.   
     
     
         10 . A method for proliferating lymphocytes
 which comprises bringing a body fluid into contact with the porous material according to  claim 1  and cultivating lymphocytes using the body fluid after contacting with said porous material.   
     
     
         11 . The method for proliferating lymphocytes according to  claim 10 ,
 wherein said body fluid is an autologous body fluid of a mammal and said mammal is in a condition of poor lymphocyte proliferation.   
     
     
         12 . A method for producing mammalian lymphocytes
 which comprises bringing a mammalian body fluid into contact with the porous material according to  claim 1 , cultivating lymphocytes using the body fluid after contacting with the porous material and recovering the thus-produced lymphocytes.   
     
     
         13 . A method for producing a pharmaceutical composition
 which comprises producing lymphocytes according to the method according to  claim 12  and blending the lymphocytes with a pharmaceutically acceptable additive.   
     
     
         14 . An additive body fluid to be added to the culture medium on the occasion of lymphocyte culture
 which is obtained by bringing a mammalian body fluid into contact with the porous material according to  claim 1 .   
     
     
         15 . A method for treating a disease against which a therapeutic effect is obtained by returning extracorporeally activated mammalian lymphocytes into the body
 which comprises bringing a body fluid derived from a mammal requiring or not requiring treatment into contact with the porous material according to  claim 1 , cultivating lymphocytes using the body fluid after contacting with the porous material, and administering the thus-obtained lymphocytes to said mammal.   
     
     
         16 . The use of activated carbon or a high-molecular compound having an angle of contact with water within the range of 40° to 98° in producing a porous material for body fluid treatment to promote the lymphocyte proliferation in lymphocyte culture. 
     
     
         17 . The porous material according to  claim 2 ,
 wherein the high-molecular compound is an aromatic high-molecular compound.   
     
     
         18 . The porous material according to  claim 2   wherein the high-molecular compound contains no amine residue bound thereto.   
     
     
         19 . The porous material according to  claim 3   wherein the high-molecular compound contains no amine residue bound thereto.   
     
     
         20 . The porous material according to  claim 4   wherein the high-molecular compound contains no amine residue bound thereto.