Factor Taking Part in Transcription Control

Abstract

HDART binds with HDAC (histone deacetylase) and function as a repressor. HDART directly binds with Skip functioning as a transcription co-activator of a nuclear receptor to repress the transcription of the nuclear receptor. Moreover, HDART is one of transcription co-repressors of nuclear receptor, and binds with HDAC thereby enabling intense repression of transcription through the histone deacetylization of HDAC. On the other hand, a dominant negative peptide of HDART can be obtained, and it has been confirmed that this peptide activates transcription contrary to the HDART protein of the full length. Especially, the transcription activity of retinoic acid receptor with this peptide has an activity exceeding all-trans retinoic acid (ATRA).

1 . DNA coded with a transcription repressing factor including:
 (A) DNA coded with a protein having an amino acid sequence indicated in Sequence No. 2; or   (B) DNA consisting of a base sequence indicated in Sequence No. 1.   
     
     
         2 . DNA coded with a transcription repressing factor including:
 (A) DNA coded with a protein having such an amino acid sequence that one or plural amino acids are substituted with, deleted from, inserted into and/or added to an amino acid sequence indicated in Sequence No. 2; or   (B) DNA hybridized with DNA consisting of a base sequence indicated in Sequence No. 1 under stringent conditions.   
     
     
         3 . The transcription repressing factor coded with DNA as set forth in  claim 1  or  2 . 
     
     
         4 . The transcription repressing factor as set forth in  claim 3  wherein transcription ascribed to a nuclear hormone receptor is repressable. 
     
     
         5 . DNA coded with a peptide capable of activating transcription including:
 (A) DNA coded with a peptide consisting of an amino acid sequence from position 1 to 179 in Sequence No. 2; or   (B) DNA consisting of a base sequence of from 1 to 537 bases in Sequence No. 1.   
     
     
         6 . DNA coded with a peptide capable of activating transcription including:
 (A) DNA coded with a peptide having such an amino acid sequence that one or plural amino acids are substituted with, deleted from, inserted into and/or added to an amino acid sequence position 1 to 179 in Sequence No. 2; or   (B) DNA hybridized with DNA consisting of a base sequence position 1 to 537 bases in Sequence No. 1 under stringent conditions.   
     
     
         7 . The transcription activation peptide coded with the DNA as set forth in  claim 5  or  6 . 
     
     
         8 . DNA as set forth in  claim 1 ,  2 ,  5  or  6  and having at least 15 nucleotide length. 
     
     
         9 . A vector inserted with the DNA as set forth in  claim 1 ,  2 ,  5  or  6 . 
     
     
         10 . A host cell holding the DNA as set forth in  claim 1 ,  2 ,  5  or  6 . 
     
     
         11 . An antibody capable of binding with the factor as set forth in  claim 3 . 
     
     
         12 . An oligonucleotide probe hybridized with the DNA as set forth in  claim 1 ,  2 ,  5  or  6  and having at least 10 nucleotide length. 
     
     
         13 . A substrate fixed with the oligonucleotide probe as set forth in  claim 12 . 
     
     
         14 . An antibody capable of binding with the peptide as set forth in  claim 7 . 
     
     
         15 . A substrate fixed with the transcription repressing factor as set forth in  claim 3 , or a partial peptide of said factor. 
     
     
         16 . A substrate fixed with the transcription activating factor as set forth in  claim 3 , or a partial peptide of said factor. 
     
     
         17 . A substrate fixed with the antibody as set forth in  claim 11 .