Blood type checking reagent containing lectin

Abstract

Lectin for checking a blood type is extracted from seeds of bitter gourd, balsam pear, or Momordica charantia . A blood type checking reagent includes the lectin. The lectin extracted from seeds of bitter gourd preferably has a molecular weight between 100,000 and 170,000 measured with poly-acrylamide gel electrophoresis in existence of sodium dodecyl sulfate (SDS-PAGE). A method of checking a blood type includes the steps of: extracting lectin from seeds of bitter gourd, balsam pear, or Momordica charantia ; preparing a blood type checking reagent containing the lectin; and checking a blood type using the blood type checking reagent.

1 . A blood type checking reagent, comprising lectin extracted from seeds of bitter gourd (Momordica charantia) through the steps of:
 grinding the seeds of bitter gourd to obtain first power;   processing the first powder to obtain second powder;   adding the second powder to normal saline solution containing gelatin to obtain a mixture; and   centrifuging the mixture to obtain supernatant as the lectin.   
     
     
         2 . The blood type checking reagent according to  claim 1 , further comprising the steps of:
 adding the first powder into distilled water to obtain a first mixture;   filtering the first mixture to obtain filtrate;   centrifuging the filtrate to obtain first supernatant;   adding the first supernatant to ethanol to obtain a second mixture;   removing second supernatant of the second mixture to obtain first deposit;   centrifuging the first deposit to obtain second deposit; and   drying the second deposit to obtain the second powder.   
     
     
         3 . The blood type checking reagent according to  claim 1 , wherein said lectin has a molecular weight between 100,000 and 170,000 measured with poly-acrylamide gel electrophoresis in existence of sodium dodecyl sulfate (SDS-PAGE).