Family 44 xyloglucanases

Abstract

The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus , especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.

1 . An isolated xyloglucanase, which is any of
 (a) a polypeptide having an amino acid sequence that is at least 80% identical with one or more of the sequences of amino acids 40-559 of SEQ ID NO: 2, 4 or 6; and   (b) a polypeptide encoded by a DNA sequence that hybridizes to one or more of nucleotides 121-1677 of SEQ ID NO: 1, 3 or 5, under medium stringency conditions, wherein the medium stringency conditions are defined as hybridization in 5×SSC at 45° C. and washing in 2×SSC at 60° C.   
     
     
         2 . The xyloglucanase of  claim 1 , which has an amino acid sequence that is at least 85% identical with amino acids 40-559 of SEQ ID NO: 2. 
     
     
         3 . The xyloglucanase of  claim 2 , which has an amino acid sequence that is at least 90% identical with amino acids 40-559 of SEQ ID NO: 2. 
     
     
         4 . The xyloglucanase of  claim 1 , which has an amino acid sequence that is at least 85% identical with amino acids 40-559 of SEQ ID NO: 4. 
     
     
         5 . The xyloglucanase of  claim 4 , which has an amino acid sequence that is at least 90% identical with amino acids 40-559 of SEQ ID NO: 4. 
     
     
         6 . The xyloglucanase of  claim 1 , which has an amino acid sequence that is at least 85% identical with amino acids 40-559 of SEQ ID NO: 6. 
     
     
         7 . The xyloglucanase of  claim 6 , which has an amino acid sequence that is at least 90% identical with amino acids 40-559 of SEQ ID NO: 6. 
     
     
         8 . The xyloglucanase of  claim 1 , which is encoded by a DNA sequence that hybridizes to nucleotides 121-1677 of SEQ ID NO: 1 under medium stringency conditions. 
     
     
         9 . The xyloglucanase of  claim 1 , which is encoded by a DNA sequence that hybridizes to nucleotides 121-1677 of SEQ ID NO: 3 under medium stringency conditions. 
     
     
         10 . The xyloglucanase of  claim 1 , which is encoded by a DNA sequence that hybridizes to nucleotides 121-1677 of SEQ ID NO: 5 under medium stringency conditions. 
     
     
         11 . The xyloglucanase of  claim 8 , which is obtained from the  Bacillus/Lactobacillus  subdivision. 
     
     
         12 . The xyloglucanase of  claim 11 , which is obtained from a species of  Paenibacillus.    
     
     
         13 . The xyloglucanase of  claim 12 , which is obtained from  Paenibacillus polymyxa.    
     
     
         14 . The xyloglucanase of  claim 9 , which is obtained from the  Bacillus/Lactobacillus  subdivision. 
     
     
         15 . The xyloglucanase of  claim 14 , which is obtained from a species of  Paenibacillus.    
     
     
         16 . The xyloglucanase of  claim 15 , which is obtained from  Paenibacillus polymyxa.    
     
     
         17 . The xyloglucanase of  claim 10 , which is obtained from the  Bacillus/Lactobacillus subdivision.    
     
     
         18 . The xyloglucanase of  claim 17 , which is obtained from a species of  Paenibacillus.    
     
     
         19 . The xyloglucanase of  claim 18 , which is obtained from  Paenibacillus polymyxa.    
     
     
         20 . A detergent composition comprising a xyloglucanase of  claim 1  and a surfactant. 
     
     
         21 . A process for washing a fabric, comprising treating the fabric during a washing cycle of a machine washing process with a washing solution which comprises a xyloglucanase of  claim 1 .