Compositions Comprising Viruses and Methods for Concentrating Virus Preparations

Abstract

A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 20° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

1 . A composition comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. 
     
     
         2 . The composition of  claim 1  wherein the virus is a recombinant virus. 
     
     
         3 . The composition of  claim 1  wherein the polyhydroxy hydrocarbon is glycerol. 
     
     
         4 . The composition of  claim 1  further comprising a disaccharide. 
     
     
         5 . The composition of  claim 1  wherein the composition comprises a buffer system comprising sodium phosphate monobasic dihydrate in a concentration of about 0.5 to 10 mg/mL and tromethamine in a concentration of about 0.5 to 10 mg/mL. 
     
     
         6 . The composition of  claim 1  further comprising a divalent metal salt in a concentration of about 0.1 to 1 mg/mL. 
     
     
         7 . The composition of  claim 1  which further comprises a diluent comprising water. 
     
     
         8 . The composition of  claim 1  wherein the virus is present in a concentration of about 1×10 9  to 1×10 13  particles/mL. 
     
     
         9 . The composition of  claim 1  wherein the virus is adenovirus. 
     
     
         10 . The composition of  claim 2  wherein the recombinant virus comprises a wild-type p53 gene. 
     
     
         11 . The composition of  claim 10  wherein the recombinant virus is A/C/N/53. 
     
     
         12 . The composition of  claim 1  wherein the virus is adenovirus; the polyhydroxy hydrocarbon is glycerol; the buffer system maintains the pH in a range from about 7.3 to about 7.9 at a temperature ranging from 20° C. to 27° C.; and the composition further comprises a disaccharide. 
     
     
         13 . The composition of  claim 12  wherein the adenovirus is A/C/N/53; the disaccharide is sucrose; the buffer system comprises sodium phosphate monobasic dihydrate and tromethamine; and the composition further comprises a divalent metal salt and water. 
     
     
         14 - 28 . (canceled)