Mouse cytokine receptor zcytor16

Abstract

Cytokines and their receptors have proven usefulness in both basic research, animal models, and as therapeutics. The present invention provides polynucleotides encoding a new cytokine receptor designated as “mouse Zcytor16”, which can bind and antagonize the IL-TIF cytokine. The present invention also provides corresponding vectors, cells, and methods of production.

1 . An isolated polynucleotide comprising the nucleic acid sequence from the group consisting of:
 (a) the polynucleotide sequence from 8 to 697 of SEQ ID NO:47;   (b) the polynucleotide sequence from 77 to 697 of SEQ ID NO:47;   (c) the polynucleotide sequence from 86 to 697 of SEQ ID NO:47; and   (d) polynucleotide sequences complementary to the polynucleotides encoding the polypeptides consisting of a sequence of amino acid residues as shown in (a), (b), and (c).   
     
     
         2 . An isolated polynucleotide of  claim 1 , wherein the polynucleotide consists of the nucleic acid sequence from the group consisting of:
 (a) the polynucleotide sequence from 8 to 697 of SEQ ID NO:47;   (b) the polynucleotide sequence from 77 to 697 of SEQ ID NO:47;   (c) the polynucleotide sequence from 86 to 697 of SEQ ID NO:47; and   (d) polynucleotide sequences complementary to the polynucleotides encoding the polypeptides consisting of a sequence of amino acid residues as shown in (a), (b), and (c).   
     
     
         3 . An isolated polynucleotide encoding a polypeptide comprising the sequence of amino acid residues selected from the group consisting of:
 (a) amino acid residues 27 to 230 of SEQ ID NO:48;   (b) amino acid residues 27 to 126 of SEQ ID NO:48; and   (c) amino acid residues 131 to 230 of SEQ ID NO:48.   
     
     
         4 . An isolated polynucleotide of  claim 3 , wherein the polynucleotide encodes the polypeptide consisting of a sequence of amino acid residues selected from the group consisting of:
 (a) amino acid residues 27 to 230 of SEQ ID NO:48;   (b) amino acid residues 27 to 126 of SEQ ID NO:48; and   (c) amino acid residues 131 to 230 of SEQ ID NO:48.   
     
     
         5 . An isolated polynucleotide encoding a soluble cytokine receptor polypeptide comprising a sequence of amino acid residues as shown from amino acid 27 to 230 of SEQ ID NO:48. 
     
     
         6 . A vector, comprising the isolated polynucleotide of  claim 3 . 
     
     
         7 . An expression vector, comprising the isolated polynucleotide of  claim 3 , a transcription promoter, and a transcription terminator, wherein the promoter is operably linked with the polynucleotide, and wherein the polynucleotide is operably linked with the transcription terminator. 
     
     
         8 . A recombinant host cell comprising the expression vector of  claim 7 , wherein the host cell is selected from the group consisting of bacterium, yeast cell, fungal cell, insect cell, mammalian cell, and plant cell. 
     
     
         9 . A method of producing a polypeptide, the method comprising culturing recombinant host cells that comprise the expression vector of  claim 7 , and that produce the polypeptide. 
     
     
         10 . The method of  claim 9 , further comprising isolating the polypeptide from the cultured recombinant host cells. 
     
     
         11 . An isolated polynucleotide that encodes the soluble cytokine receptor polypeptide of  claim 5 , wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide sequence binds IL-10-Related T Cell-Derived Inducible Factor (IL-TIF) or antagonizes IL-TIF activity. 
     
     
         12 . The isolated polynucleotide according to  claim 11 , wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide comprises a homodimeric, heterodimeric or multimeric receptor complex. 
     
     
         13 . The isolated polynucleotide according to  claim 12 , wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide comprises a heterodimeric or multimeric receptor complex further comprising a soluble Class I or Class II cytokine receptor. 
     
     
         14 . The isolated polynucleotide according to  claim 12 , wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide comprises a heterodimeric or multimeric receptor complex further comprising a soluble Cytokine Receptor Family 2-4 (CRF2-4) receptor polypeptide (SEQ ID NO:35), a soluble IL-10 receptor polypeptide (SEQ ID NO:36), or soluble zcytor11 receptor polypeptide (SEQ ID NO:34). 
     
     
         15 . An isolated polynucleotide that encodes the soluble cytokine receptor polypeptide of  claim 5 , wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide comprises a homodimeric, heterodimeric or multimeric receptor complex. 
     
     
         16 . An isolated polynucleotide according to  claim 15 , wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide further comprises a soluble Class I or Class II cytokine receptor. 
     
     
         17 . An isolated polynucleotide according to  claim 15 , wherein the soluble cytokine receptor polypeptide encoded by the polynucleotide comprises a heterodimeric or multimeric receptor complex further comprising a soluble Cytokine Receptor Family 2-4 (CRF2-4) receptor polypeptide (SEQ ID NO:35), a soluble IL-10 receptor polypeptide (SEQ ID NO:36), or soluble zcytor11 receptor polypeptide (SEQ ID NO:34). 
     
     
         18 . An isolated polynucleotide according to  claim 15 , wherein the soluble cytokine receptor polypeptide further encodes an intracellular domain. 
     
     
         19 . An isolated polynucleotide according to  claim 15 , wherein the soluble cytokine receptor polypeptide further comprises an affinity tag. 
     
     
         20 . An expression vector comprising the following operably linked elements:
 (a) a transcription promoter; a first DNA segment encoding the soluble cytokine receptor polypeptide of  claim 5 ; and a transcription terminator; and   (b) a second transcription promoter; a second DNA segment encoding a soluble Class I or Class II cytokine receptor polypeptide; and a transcription terminator; and   wherein the first and second DNA segments are contained within a single expression vector or are contained within independent expression vectors.   
     
     
         21 . An expression vector according to  claim 20 , further comprising a secretory signal sequence operably linked to the first and second DNA segments. 
     
     
         22 . An expression vector according to  claim 20 , wherein the second DNA segment encodes a polypeptide comprising a soluble Cytokine Receptor Family 2-4 (CRF2-4) receptor polypeptide (SEQ ID NO:35), a soluble IL-10 receptor polypeptide (SEQ ID NO:36), or soluble zcytor11 receptor polypeptide (SEQ ID NO:34). 
     
     
         23 . A cultured cell comprising an expression vector according to  claim 20 , wherein the cell expresses the polypeptides encoded by the DNA segments. 
     
     
         24 . A cultured cell comprising an expression vector according to  claim 20 , wherein the first and second DNA segments are located on independent expression vectors and are co-transfected into the cell, and cell expresses the polypeptides encoded by the DNA segments. 
     
     
         25 . A cultured cell into which has been introduced an expression vector according to  claim 20 , wherein the cell expresses a heterodimeric or multimeric soluble receptor polypeptide encoded by the DNA segments. 
     
     
         26 . A cell according to  claim 23 , wherein the cell secretes a soluble cytokine receptor polypeptide heterodimer or multimeric complex. 
     
     
         27 . A cell according to  claim 23 , wherein the cell secretes a soluble cytokine receptor polypeptide heterodimer or multimeric complex that binds IL-10-Related T Cell-Derived Inducible Factor (IL-TIF) or antagonizes IL-TIF activity. 
     
     
         28 . A DNA construct encoding a fusion protein comprising:
 a first DNA segment encoding the soluble cytokine receptor polypeptide of  claim 5 ; and   at least one other DNA segment encoding a soluble Class I or Class II cytokine receptor polypeptide,   wherein the first and other DNA segments are connected in-frame; and   wherein the first and other DNA segments encode the fusion protein.   
     
     
         29 . A DNA construct encoding a fusion protein according to  claim 28 , wherein at least one other DNA segment encodes a polypeptide comprising a soluble Cytokine Receptor Family 2-4 (CRF2-4) receptor polypeptide (SEQ ID NO:35), a soluble IL-10 receptor polypeptide (SEQ ID NO:36), or soluble zcytor11 receptor polypeptide (SEQ ID NO:34). 
     
     
         30 . An expression vector comprising the following operably linked elements:
 a transcription promoter;   a DNA construct encoding a fusion protein according to  claim 28 ; and   a transcription terminator,   wherein the promoter is operably linked to the DNA construct, and the DNA construct is operably linked to the transcription terminator.   
     
     
         31 . A cultured cell comprising an expression vector according to  claim 30 , wherein the cell expresses a polypeptide encoded by the DNA construct. 
     
     
         32 . A method of producing a fusion protein comprising:
 culturing a cell according to  claim 31 ; and   isolating the polypeptide produced by the cell.   
     
     
         33 . A method of producing a soluble cytokine receptor polypeptide that comprises a heterodimeric or multimeric complex comprising:
 culturing a cell according to  claim 23 ; and   isolating the soluble receptor polypeptides produced by the cell.   
     
     
         34 . The isolated polynucleotide according to  claim 15 , wherein the soluble cytokine receptor polypeptide further comprises an affinity tag, chemical moiety, toxin, label, biotin/avidin label, radionuclide, enzyme, substrate, cofactor, inhibitor, fluorescent marker, chemiluminescent marker, cytotoxic molecule or an immunoglobulin Fc domain. 
     
     
         35 . The isolated polynucleotide according to  claim 3 , wherein the polypeptide by the polynucleotide further comprises an affinity tag, chemical moiety, toxin, label, biotin/avidin label, radionuclide, enzyme, substrate, cofactor, inhibitor, fluorescent marker, chemiluminescent marker, cytotoxic molecule or an immunoglobulin Fc domain. 
     
     
         36 . The isolated polynucleotide of  claim 3 , wherein the polypeptide encoded by the polynucleotide binds IL-10-Related T Cell-Derived Inducible Factor (IL-TIF) or antagonizes IL-TIF activity.