Method for releasing genetic material from solid phase

Abstract

The present invention relates to systems for releasing genetic materials from a solid medium. The present invention also relates to methods for releasing genetic materials from a solid medium. The present invention further relates to methods for isolating genetic material from a biological sample.

1 . A genetic material releasing system comprising:
 a pressure wave generating device, a chamber and a solid medium in the chamber, wherein the solid medium is configured to adsorb or bind genetic material from a sample, the pressure wave generating device is configured to emit pressure waves, and the solid medium and the chamber are configured so that when a portion of the pressure waves impinges upon the solid medium, a portion of the adsorbed or bound genetic material is released from the solid medium.   
     
     
         2 . The genetic material releasing system of  claim 1 , wherein the pressure wave generating device is a sound wave generating device. 
     
     
         3 . The genetic material releasing system of  claim 2 , wherein the sound wave generating device is an acoustic energy emitting device. 
     
     
         4 . The genetic material releasing system of  claim 3 , wherein the acoustic energy emitting device is an ultrasonic transducer, a piezoelectric transducer, a magnorestrictive transducer or an electrostatic transducer. 
     
     
         5 . The genetic material releasing system of  claim 3 , wherein the acoustic energy emitting device is inserted into a liquid in contact with the solid medium. 
     
     
         6 . The genetic material releasing system of  claim 5 , wherein the acoustic energy emitting device is a sonicator. 
     
     
         7 . The genetic material releasing system of  claim 3 , wherein the chamber is inserted into a liquid in contact with the acoustic energy emitting device. 
     
     
         8 . The genetic material releasing system of  claim 7 , wherein the acoustic energy emitting device is a transducer that focuses acoustic energy. 
     
     
         9 . The genetic material releasing system of  claim 8 , wherein the acoustic energy emitting device is a dish-shaped transducer. 
     
     
         10 . The genetic material releasing system of  claim 3 , wherein the acoustic energy emitting device is in direct contact with the chamber. 
     
     
         11 . The genetic material releasing system of  claim 1 , wherein the solid medium is selected from the group consisting of filter paper, silica particles, silica gel particles, glass particles, glass fibers, glass microfibers, glass fiber fleece, polymeric materials, cellulosic materials, metallic beads, magnetic beads, metallic particles and magnetic particles. 
     
     
         12 . The genetic material releasing system of  claim 11 , wherein the cellulosic material is a cellulose based substrate. 
     
     
         13 . The genetic releasing system of  claim 1  which further comprises a mechanical energy device, wherein solid medium and the chamber are configured so that when a portion of the mechanical energy impinges upon the solid medium, a portion of the adsorbed or bound genetic material is released from the solid medium. 
     
     
         14 . A method of releasing genetic material from a solid medium having the genetic material adsorbed or bound thereto, said method comprising subjecting a solid medium having genetic material adsorbed or bound thereto to a pressure wave to release at least a portion of the genetic material from the solid medium. 
     
     
         15 . The method of  claim 14 , wherein the pressure wave is a sound wave. 
     
     
         16 . The method of  claim 15 , wherein the sound wave is produced by an acoustic energy emitting device. 
     
     
         17 . The method of  claim 16 , wherein the acoustic energy emitting device is an ultrasonic transducer, a piezoelectric transducer, a magnorestrictive transducer or an electrostatic transducer. 
     
     
         18 . The method of  claim 16 , wherein the solid medium is subjected to the acoustic energy by inserting an acoustic energy emitting device into a liquid in contact with the solid medium. 
     
     
         19 . The method of  claim 18 , wherein the solid medium is subjected to the acoustic energy by inserting a sonicator into a liquid in contact with the solid medium. 
     
     
         20 . The method of  claim 16 , wherein the solid medium is subjected to the acoustic energy by inserting a chamber holding the solid medium into a liquid in contact with an acoustic energy emitting device. 
     
     
         21 . The method of  claim 20 , wherein the solid medium is subjected to the acoustic energy by inserting a chamber holding the solid medium into a liquid in contact with an transducer that focuses acoustic energy. 
     
     
         22 . The method of  claim 21 , wherein the solid medium is subjected to the acoustic energy by inserting a chamber holding the solid medium into a liquid in contact with a dish-shaped transducer. 
     
     
         23 . The method of  claim 16 , wherein the acoustic energy emitting device is in direct contact with the chamber. 
     
     
         24 . The method of  claim 14 , wherein the solid medium is selected from the group consisting of filter paper, silica particles, silica gel particles, glass particles, glass fibers, glass microfibers, glass fiber fleece, polymeric materials, cellulosic materials, metallic beads, magnetic beads, metallic particles and magnetic particles. 
     
     
         25 . The method of  claim 24 , wherein the cellulosic material is a cellulose based substrate. 
     
     
         26 . The method of  claim 14 , which further comprises subjecting the solid medium to mechanical energy to release at least a portion of the genetic material from the solid medium. 
     
     
         27 . The method of  claim 26 , wherein the solid medium is subjected to the mechanical energy and the pressure wave at the same time. 
     
     
         28 . A method of recovering genetic material from a biological sample which comprises the steps of:
 (a) contacting a biological sample comprising genetic material with a solid medium;   (b) retaining the genetic material with the solid medium;   (c) subjecting the solid medium with retained genetic material to a pressure wave to release at least a portion of the genetic material from the solid medium; and   (d) recovering the released genetic material.   
     
     
         29 . The method of  claim 28 , wherein the pressure wave is a sound wave. 
     
     
         30 . The method of  claim 29 , wherein the sound wave is produced by an acoustic energy emitting device. 
     
     
         31 . The method of  claim 30 , wherein the acoustic energy emitting device is an ultrasonic transducer, a piezoelectric transducer, a magnorestrictive transducer or an electrostatic transducer. 
     
     
         32 . The method of  claim 30 , wherein the solid medium is subjected to the acoustic energy by inserting an acoustic energy emitting device into a liquid in contact with the solid medium. 
     
     
         33 . The method of  claim 32 , wherein the solid medium is subjected to the acoustic energy by inserting a sonicator into a liquid in contact with the solid medium. 
     
     
         34 . The method of  claim 30 , wherein the solid medium is subjected to the acoustic energy by inserting a chamber holding the solid medium into a liquid in contact with an acoustic energy emitting device. 
     
     
         35 . The method of  claim 34 , wherein the solid medium is subjected to the acoustic energy by inserting a chamber holding the solid medium into a liquid in contact with a transducer that focuses acoustic energy. 
     
     
         36 . The method of  claim 35 , wherein the solid medium is subjected to the acoustic energy by inserting a chamber holding the solid medium into a liquid in contact with a dish-shaped transducer. 
     
     
         37 . The method of  claim 30 , wherein the acoustic energy emitting device is in direct contact with the chamber. 
     
     
         38 . The method of  claim 30 , wherein the acoustic energy emitting device is positioned sufficiently close to the chamber such that the acoustic energy emitted from the acoustic energy emitting device causes the release of at least a portion of genetic material from the solid medium. 
     
     
         39 . The method of  claim 28 , wherein the solid medium is selected from the group consisting of filter paper, silica particles, silica gel particles, glass particles, glass fibers, glass microfibers, glass fiber fleece, polymeric materials, cellulosic materials, metallic beads, magnetic beads, metallic particles and magnetic particles. 
     
     
         40 . The method of  claim 39 , wherein the cellulosic material is a cellulose based substrate. 
     
     
         41 . The method of  claim 28 , wherein the biological sample comprises cells containing the genetic material and the cells are lysed before, while or after contacting the biological sample with the solid medium. 
     
     
         42 . The method of  claim 41 , wherein the solid medium is selected from the group consisting of filter paper, silica particles, silica gel particles, glass particles, glass fibers, glass microfibers, glass fiber fleece, polymeric materials, cellulosic materials, metallic beads, magnetic beads, metallic particles and magnetic particles. 
     
     
         43 . The method of  claim 42 , wherein the cellulosic material is a cellulose based substrate. 
     
     
         44 . The method of  claim 28 , wherein the biological sample comprises cells containing the genetic material and the solid medium is capable of lysing the cells. 
     
     
         45 . The method of  claim 44 , wherein the solid medium is selected from the group consisting of filter paper, silica particles, silica gel particles, glass particles, glass fibers, glass microfibers, glass fiber fleece, polymeric materials, cellulosic materials, metallic beads, magnetic beads, metallic particles and magnetic particles. 
     
     
         46 . The method of  claim 45 , wherein the cellulosic material is a cellulose based substrate. 
     
     
         47 . The method of  claim 28 , which further comprises subjecting the solid medium to mechanical energy. 
     
     
         48 . The method of  claim 47 , wherein the solid medium is subjected to the mechanical energy and the pressure wave at the same time. 
     
     
         49 . A nucleic acid releasing device comprising:
 an energy emitting device;   a chamber; and   an adsorption substrate, wherein the absorption substrate is within the chamber, wherein the absorption substrate is configured to adsorb nucleic acid from a sample, and wherein the energy emitting device is configured to emit energy, wherein the adsorption substrate and chamber are configured so that when a portion of the energy impinges upon the adsorption substrate, a portion of the adsorbed nucleic acid is released from the adsorption substrate.   
     
     
         50 . The nucleic acid releasing device according to  claim 49 , wherein the energy is contained in pressure waves. 
     
     
         51 . The nucleic acid releasing device according to  claim 50 , wherein the pressure waves are sound waves. 
     
     
         52 . The nucleic acid releasing device according to  claim 51 , wherein the sound waves are produced by an acoustic energy emitting device. 
     
     
         53 . The nucleic acid releasing device according to  claim 52 , wherein the acoustic energy emitting device is an ultrasonic transducer, a piezoelectric transducer, a magnorestrictive transducer or an electrostatic transducer. 
     
     
         54 . The nucleic acid releasing device according to  claim 52 , wherein the acoustic energy emitting device is inserted into a liquid in contact with the adsorption substrate. 
     
     
         55 . The nucleic acid releasing device according to  claim 54 , wherein the acoustic energy emitting device is a sonicator. 
     
     
         56 . The nucleic acid releasing device according to  claim 52 , wherein the chamber is inserted into a liquid in contact with the acoustic energy emitting device. 
     
     
         57 . The nucleic acid releasing device according to  claim 56 , wherein the acoustic energy emitting device is a transducer that focuses acoustic energy. 
     
     
         58 . The nucleic acid releasing device according to  claim 57 , wherein the acoustic energy emitting device is a dish-shaped transducer. 
     
     
         59 . The nucleic acid releasing device according to  claim 52 , wherein the acoustic energy emitting device is in direct contact with the chamber. 
     
     
         60 . The nucleic acid releasing device according to  claim 52 , wherein the acoustic energy emitting device is positioned sufficiently close to the chamber such that the acoustic energy emitted from the acoustic energy emitting device causes the release of at least a portion of genetic material from the solid medium. 
     
     
         61 . The nucleic acid releasing device according to  claim 49 , wherein the energy is contained in thermal energy. 
     
     
         62 . The genetic releasing system of  claim 49  which further comprises a mechanical energy device, wherein the solid medium and the chamber are configured so that when a portion of the mechanical energy impinges upon the solid medium, a portion of the adsorbed or bound nucleic acid is released from the solid medium.