Methods and apparatus for carrier-free deflection electrophoresis

Abstract

The invention relates to a method and apparatus for carrier-free deflection electrophoresis, in which a separating media and a sample to be examined flow through a separating chamber between a pair of electrodes in a series of reversing bulk fluid flow along the direction of the electrodes, thereby separating the sample into zones which are to be collected into fractions for analysis or further processing. Among other things, the apparatus and method enable high-resolution separation of particles that can be performed in miniaturized chambers in electrophoresis modes including isoelectric focusing, zone electrophoresis, and isotachophoresis.

1 . A method for separating particles comprising:
 disposing a separation medium and sample in an electrophoresis chamber having a top plate, a bottom plate and a plurality of electrodes generally parallel to one another with a separation space disposed therebetween, and a fluidic displacement system for conveying a separation medium between the electrodes;   applying a voltage between the electrodes effective to manipulate particles electrophoretically;   displacing at least a portion of the separation medium and sample towards a first direction generally parallel to the direction of the electrodes;   displacing at least a portion of the separation medium and sample in a second direction generally opposite the first direction.   
     
     
         2 . The method of  claim 1 , further comprising the step of eluting the separation medium and sample from the electrophoresis chamber through one or more outlets. 
     
     
         3 . The method of  claim 2 , wherein at least a portion of the separation medium and the sample is eluted from the separation space into individual collection cavities such as vessels or wells in microtiter plates. 
     
     
         4 . The method of  claim 2 , wherein at least a portion of the separation medium and sample are eluted towards outlets by displacing the separation medium and sample in a direction consistent with the first or second direction. 
     
     
         5 . The method of  claim 2 , wherein prior to eluting the separation medium and sample from the electrophoresis chamber, the method comprises removing the voltage between the electrodes effective to manipulate particles electrophoretically. 
     
     
         6 . The method of  claim 1 , wherein applying a voltage between the electrodes enables an electrophoretic separation mode chosen from the group consisting of isoelectric focusing, isotachophoresis, and zone electrophoresis. 
     
     
         7 . The method of  claim 1 , wherein the top plate and bottom plate are stationary with respect to the electrodes and each other while applying the voltage. 
     
     
         8 . The method of  claim 1 , wherein one cycle comprises
 displacing the separation medium and sample towards a first direction generally parallel to the direction of the electrodes, and   displacing the separation medium and sample in a second direction generally opposite the first direction;   and wherein the number of cycles is greater than one.   
     
     
         9 . The method of  claim 8 , wherein the number of cycles is selected so as to achieve sufficient separation of the sample. 
     
     
         10 . The method of  claim 1 , further comprising the step of introducing and displacing a counterflow medium into the separation space. 
     
     
         11 . An apparatus for separating particles comprising:
 an electrophoresis chamber having a top plate, a bottom plate and a plurality of electrodes generally parallel to one another with a separation space disposed therebetween,   a fluidic displacement system configured to introduce a separation medium and to introduce a sample between the electrodes; and   a second fluidic displacement system configured to cyclically displace the separation medium and sample between a first direction generally parallel to the direction of the electrodes and a second direction generally opposite the first direction.   
     
     
         12 . The apparatus of  claim 11 , wherein the top plate and bottom plate are stationary with respect to the electrodes and each other. 
     
     
         13 . The apparatus of  claim 11 , wherein the separation space between the top plate and bottom plate comprises a thickness of about 0.01 to about 1.5 mm. 
     
     
         14 . The apparatus of  claim 11 , wherein the first fluidic displacement system comprises a multi-channel pump. 
     
     
         15 . The apparatus of  claim 11 , wherein the fluidic displacement system for introducing a separation medium and introducing a sample between the electrodes is further used to remove the sample after separation from the separation space. 
     
     
         16 . The apparatus of  claim 11 , wherein the second fluidic displacement system comprises a multi-channel pump. 
     
     
         17 . The apparatus of  claim 11 , further comprising a controller configured to control the fluidic displacement systems. 
     
     
         18 . The apparatus of  claim 11 , further comprising a controller configured to control the flow of current between the electrodes. 
     
     
         19 . An apparatus for separating particles comprising:
 an electrophoresis chamber defined by a top plate, a bottom plate and a plurality of electrodes generally parallel to one another with a separation space disposed therebetween,   a fluidic displacement system configured to introduce a separation medium and to introduce a sample between the electrodes and configured to cyclically displace the separation medium and sample between a first direction generally parallel to the direction of the electrodes and a second direction generally opposite the first direction.   
     
     
         20 . The apparatus of  claim 19 , wherein the top plate and bottom plate are stationary with respect to the electrodes and each other. 
     
     
         21 . The apparatus of  claim 19 , wherein the fluidic displacement system comprises a multi-channel pump. 
     
     
         22 . The apparatus of  claim 19 , further comprising electrode spacers configured to isolate the electrodes from the separation space. 
     
     
         23 . The apparatus of  claim 19 , further comprising a fraction collector outlet configured to displace the separated fractions of the sample from the separation space into individual collection cavities such as vessels or wells in microtiter plates. 
     
     
         24 . A method for separating particles comprising:
 disposing a separation medium and sample in an electrophoresis chamber having a top plate, a bottom plate, a first chamber end, a second chamber end, and a plurality of electrodes generally parallel to one another with a separation space disposed therebetween, the electrodes longitudinally extending toward each of the ends, and a fluidic displacement system for conveying a separation medium between the first and second chamber ends;   applying a voltage between the electrodes effective to manipulate particles electrophoretically;   displacing at least a portion of the separation medium and sample towards the first chamber end; and   displacing at least a portion of the separation medium and sample towards the second chamber end; and   displacing at least a portion of the separation medium and sample towards the first chamber end.   
     
     
         25 . A computer executable software code stored on a computer readable medium, the code for effectuating the separation of particles in an electrophoresis chamber, the electrophoresis chamber having a top plate, a bottom plate, and a plurality of electrodes generally parallel to one another with a separation space disposed therebetween, and a fluidic displacement system for conveying a separation medium between the electrodes, comprising:
 code to enable application of a voltage between the electrodes effective to manipulate particles electrophoretically;   code to enable displacement of at least a portion of the separation medium and sample toward a first direction parallel to the direction of the electrodes;   code to enable displacement of at least a portion of the separation medium and sample toward a second direction opposite the first direction; and   code to enable displacement of the at least a portion of the separation medium and sample toward the first direction.