Magnetic substance-biosubstance complex structure, peptide fragment capable of linking to magnetic substance and gene therefor, and process for producing the complex structure

Abstract

A magnetic substance-biosubstance complex structure comprises a magnetic substance-containing carrier and a biosubstance immobilized on the carrier, the biosubstance being immobilized through a spacer comprising an amino acid sequence on a surface of the carrier.

1 . A magnetic substance-biosubstance complex structure comprising: 
 a magnetic substance-containing carrier; and    a biosubstance immobilized on the carrier, the biosubstance being immobilized through a spacer comprising an amino acid sequence that binds to the magnetic substance on a surface of the carrier and, wherein the magnetic substance comprises an MO.Fe 2 O 3  structure (M: bivalent metal) or an Fe 2 O 3  structure, the biosubstance is one or more biosubstances selected from the group consisting of proteins, carbohydrates, lipids, and complexes thereof and the amino acid sequence is selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17 SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30: and complexes thereof.    
     
     
         2 . (canceled)  
     
     
         3 . (canceled)  
     
     
         4 . (canceled)  
     
     
         5 . (canceled)  
     
     
         6 . The structure according to  claim 1 , wherein the protein is a polyhydroxyalkanoate-synthesizing enzyme.  
     
     
         7 . (canceled)  
     
     
         8 . A peptide fragment that binds to a magnetic substance, comprising an amino acid sequence selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30.  
     
     
         9 . A DNA sequence encoding a peptide fragment that binds to a magnetic substance, the peptide fragment comprising an amino acid sequence selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30.  
     
     
         10 . A fusion type polyhydroxyalkanoate-polymerizing enzyme protein, which is a fusion type protein formed from a peptide capable of linking to a magnetic substance and a polyhydroxyalkanoate-polymerizing enzyme protein; 
 the peptide comprising a amino acid sequence selected from the group consisting of SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30; and the polyhydroxyalkanoate-polymerizing enzyme protein being SEQ ID NO:1 of SEQ ID NO:3.    
     
     
         11 . A process for producing a structure having a biosubstance immobilized on a magnetic substance-containing carrier through a spacer comprising an amino acid sequence, the process comprising: 
 (1) a step of preparing a biosubstance-spacer complex formed by linking the spacer and the biosubstance; and    (2) a step of bringing the biosubstance-spacer complex with the carrier; wherein the biosubstance is immobilized by linking a portion of the spacer of the biosubstance-spacer complex to the surface of the carrier.    
     
     
         12 . The process for producing the structure according to  claim 11 , wherein the spacer comprises a peptide structure comprising two or more amino acid units.  
     
     
         13 . The process according to  claim 12 , wherein the biosubstance contains a protein, the step of producing a biosubstance-spacer complex includes an operation of expressing a fusion type protein constituted of the peptide structure and the protein joined together according to joined gene having a base sequence formed by joining a base sequence coding amino acid sequence of the protein and a base sequence of coding the amino acid sequence of the peptide structure; 
 and the biosubstance-spacer complex is prepared by the fusion type protein.    
     
     
         14 . The process for producing the structure according to  claim 13 , wherein the protein included in the biosubstance is a polyhydroxyalkanoate-synthesizing enzyme.  
     
     
         15 . The process for producing the structure according to  claim 11 , wherein the magnetic substance comprises an MO.Fe2O3 structure (M: bivalent metal) or an Fe2O3 structure, and the amino acid sequence in the spacer is one or more of amino acid sequence and/or complexes selected from the group consisting of amino acid sequences represented by SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30; and complex thereof.