Toll/Interleukin-1 receptor adapter protein (TIRAP)

Abstract

TIRAP polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing TIRAP polypeptides and polynucleotides in therapy, and diagnostic assays for such.

1 .- 44 . (canceled)  
     
     
         45 . An isolated polynucleotide having at least 95% identity to SEQ ID NO: 2, wherein the isolated polynucleotide encodes a Toll/Interleukin-1 Receptor Adapter Protein.  
     
     
         46 . The isolated polynucleotide of  claim 45 , wherein the isolated polynucleotide is SEQ ID NO: 2 but for at least one conservative codon substitution.  
     
     
         47 . The isolated polynucleotide of  claim 45 , wherein the isolated polynucleotide is SEQ ID NO: 2.  
     
     
         48 . The isolated polynucleotide of  claim 45 , wherein the Toll/Interleukin-1 Receptor Adapter Protein is SEQ ID NO: 4.  
     
     
         49 . The isolated polynucleotide of  claim 45 , wherein the isolated polynucleotide is a component of a recombinant DNA molecule.  
     
     
         50 . The isolated polynucleotide of  claim 49 , wherein the isolated polynucleotide is SEQ ID NO: 2 but for at least one conservative codon substitution.  
     
     
         51 . The isolated polynucleotide of  claim 49 , wherein the isolated polynucleotide is SEQ ID NO: 2.  
     
     
         52 . An isolated polynucleotide encoding a Toll/Interleukin-1 Receptor Adapter Protein having at least 95% identity to SEQ ID NO: 4.  
     
     
         53 . The isolated polynucleotide of  claim 52 , wherein the Toll/Interleukin-1 Receptor Adapter Protein is SEQ ID NO: 4.  
     
     
         54 . The isolated polynucleotide of  claim 52 , wherein the isolated polynucleotide is a component of a recombinant DNA molecule.  
     
     
         55 . The isolated polynucleotide of  claim 54 , wherein the Toll/Interleukin-1 Receptor Adapter Protein is SEQ ID NO: 4.  
     
     
         56 . An expression vector comprising a polynucleotide that encodes a Toll/Interleukin-1 Receptor Adapter Protein having at least 95% identity to SEQ ID NO: 4.  
     
     
         57 . The expression vector of  claim 56 , wherein the Toll/Interleukin-1 Receptor Adapter Protein is SEQ ID NO: 4.  
     
     
         58 . The expression vector of  claim 56 , wherein the polynucleotide has at least 95% identity to SEQ ID NO: 2.  
     
     
         59 . A cell comprising an expression vector that includes a polynucleotide that encodes a Toll/Interleukin-1 Receptor Adapter Protein having at least 95% identity to SEQ ID NO: 4.  
     
     
         60 . The cell of  claim 59 , wherein the Toll/Interleukin-1 Receptor Adapter Protein is SEQ ID NO: 4.  
     
     
         61 . The cell of  claim 59 , wherein the polynucleotide has at least 95% identity to SEQ ID NO: 2.  
     
     
         62 . A method of making a Toll/Interleukin-1 Receptor Adapter Protein, comprising the steps of: 
 (a) culturing a cell comprising an expression vector that includes a polynucleotide that encodes a Toll/Interleukin-1 Receptor Adapter Protein having at least 95% identity to SEQ ID NO: 4; and    (b) isolating the Toll/Interleukin-1 Receptor Adapter Protein.    
     
     
         63 . The method of  claim 62 , wherein the Toll/Interleukin-1 Receptor Adaptor Protein is SEQ ID NO: 4.  
     
     
         64 . The method of  claim 62 , wherein the polynucleotide has at least 95% identity to SEQ ID NO: 2.  
     
     
         65 . The method of  claim 62 , wherein the cell is a eukaryotic cell.  
     
     
         66 . The method of  claim 62 , wherein the cell is a prokaryotic cell.