US2008090265A1PendingUtilityA1

Method to Assay Coenzzyme Q10 in Blood Plasma or Blood Serum

37
Individually held — no corporate assignee on recordPriority: May 23, 2002Filed: Dec 3, 2007Published: Apr 17, 2008
Est. expiryMay 23, 2022(expired)· nominal 20-yr term from priority
Inventors:Gian LittarruFabrizo MoscaDaniele FattoriniStefano Bompadre
G01N 33/5735
37
PatentIndex Score
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Cited by
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References
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Claims

Abstract

A method is described for determining CoQ 10 concentrations in plasma samples. CoQ 10 in the plasma sample is oxidized by treating the sample with an oxidizing agent having a redox potential higher than the redox potential of COQ 10 , such as, for example, para-benzoquinone. Following oxidation of the COQ 10 , the CoQ 10 in the plasma sample is extracted with an alcohol, such as, for example, 1-propanol. The alcohol extract is analyzed using direct injection into the HPLC apparatus. This method achieves a rapid, accurate analysis of plasma CoQ 10 levels, which can be used for monitoring the bioavailability of orally administered CoQ 10 used as a food supplement or as an adjunctive therapy.

Claims

exact text as granted — not AI-modified
1 . A method for determining the total concentration of CoQ 10  in blood plasma or blood serum, comprising the steps of: 
 (a) obtaining a sample of blood plasma or blood serum from a human;    (b) treating the sample with an oxidizing agent having a redox potential than the redox potential of the ubiquinone/ubiquinol couple    (c) extracting the ubiquinone-10 from the sample using an alcohol; and    (d) following extraction of the ubiquinone-10, analyzing the alcohol extract using High Pressure Liquid Chromatography to determine the concentration of of ubiquinone-10, in the alcohol extract.    
     
     
         2 . (canceled)  
     
     
         3 . The method of  claim 1 , wherein the alcohol is selected from the group consisting of n-propanol, butanol and pentanol.  
     
     
         4 . The method of  claim 1 , wherein the alcohol is 1-propanol.  
     
     
         5 . (canceled)  
     
     
         6 . A method for determining the total concentration of CoQ 10  in blood plasma or blood serum, comprising the steps of: 
 (a) obtaining a sample of blood plasma or blood serum from a human;    (b) depositing an aliquot of the sample in a container;    (c) adding to the sample in the container a sufficient volume of an oxidizing agent having a redox potential higher than the redox potential of ubiquinone/ubiquinol couple to oxidize ubiquinol-10 in the sample;    (d) vortexing the sample containing the oxidizing agent;    (e) allowing the vortexed sample containing the oxidizing agent to stand for a sufficient period of time for the oxidizing agent to oxidize ubiquinol-10 contained in the sample;    (f) adding an alcohol to the sample containing the oxidizing agent to extract ubiquinone-10 from the sample;    (g) vortexing the sample containing the alcohol;    (b) centrifuging the sample containing the alcohol to spin down the protein precipitate;    (i) removing an aliquot of the supernatant from the centrifuged sample and analyzing the aliquot of supernatant using High Pressure Liquid Chromatography to determine the concentration of ubiquinone-10 in the sample.    
     
     
         7 . (canceled)  
     
     
         8 . The method of  claim 6 , wherein the alcohol is selected from the group consisting of n-propanol, butanol and pentanol.  
     
     
         9 . The method of  claim 6 , wherein the alcohol is 1-propanol.  
     
     
         10 . (canceled)  
     
     
         11 . (canceled)

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