Method to simultaneously detect different antibodies and antigens in clinical alimentary and environmental samples

Abstract

Method to simultaneously detect different antibodies and antigens via immunoenzimatic tests and ELISA (Enzyme Linked ImmunoSorbent Assay) constituted by small absorbent cylinders, on which the immunocomplexes are formed, blocked at a modular distance on a probe; that carries a label to identify the sample under examination.

1 . Method to simultaneously detect different antibodies and antigen via immunoenzimatic test and ELISA—Enzyme Linked ImmunoSorbent Assay, wherein a solid phase on which an immunocomplex forms is constituted by adsorbent cylinders disposed on a rod; said adsorbent cylinders are placed on the rod and sensitized with different reagent, immerging them into microwells of a microplate; said adsorbent cylinders of each rod once sensitized, said rod is inserted into a container with a sample to be analyzed; that once the incubation period for the sample in the container has terminated, each rod is placed on a support; said adsorbent cylinders of each rod on the support are cleaned; said adsorbent cylinders of each rod on the support are inserted into the microwells of a microplate, containing specific conjugates at a suitable temperature for a period of incubation; that, once the incubation terminates, the adsorbent cylinders on the support are lifted from the microplate and cleaned; said adsorbent cylinders from each rod on the support are inserted into the microwells of a microplate, containing a chromogenous sub layer at a suitable temperature for a period of incubation; that the small cylinders of each rod on the support are extracted and the results are read.  
     
     
         2 . Method to simultaneously detect different antibodies and antigens via the immunoenzimatic tests and ELISA ELISA—Enzyme Linked ImmunoSorbent Assay, according to  claim 1 , wherein a container for the sample to be analyzed is constituted by a microplate that has not been sensitized and that on such a microplate the grill, furnished with rods bearing the small cylinders is placed. For a suitable incubation period at the necessary temperature.