US2008032336A1PendingUtilityA1

Chemokine receptor materials and methods

62
Individually held — no corporate assignee on recordPriority: Dec 20, 1995Filed: Mar 29, 2007Published: Feb 7, 2008
Est. expiryDec 20, 2015(expired)· nominal 20-yr term from priority
Inventors:Patrick GrayVicki L. SchweickartCarol Raport
A61P 31/12A61P 7/02A61P 31/18A61P 29/00A61P 19/02A61P 17/06C07K 14/7158C07K 2319/43C07K 16/18C07K 16/2866
62
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Claims

Abstract

The present invention provides polynucleotides that encode the chemokine receptors 88-2B or 88C and materials and methods for the recombinant production of these two chemokine receptors. Also provided are assays utilizing the polynucleotides which facilitate the identification of ligands and modulators of the chemokine receptors. Receptor fragments, ligands, modulators, and antibodies are useful in the detection and treatment of disease states associated with the chemokine receptors such as atherosclerosis, rheumatoid arthritis, tumor growth suppression, asthma, viral infection, AIDS, and other inflammatory conditions.

Claims

exact text as granted — not AI-modified
1 . A purified and isolated polynucleotide encoding the amino acid sequence of chemokine receptor 88-2B set out in SEQ ID NO:4.  
   
   
       2 . A polynucleotide according to  claim 1  wherein the polynucleotide is DNA.  
   
   
       3 . A polynucleotide according to  claim 2  wherein the polynucleotide is genomic DNA.  
   
   
       4 . A polynucleotide according to  claim 2  wherein the polynucleotide is cDNA.  
   
   
       5 . A polynucleotide according to  claim 1  which is a wholly or partially chemically synthesized DNA.  
   
   
       6 . An RNA transcript of the polynucleotide of  claim 2 .  
   
   
       7 . A cDNA according to  claim 4  comprising the DNA of SEQ ID NO:3.  
   
   
       8 . A biologically functional DNA vector comprising a DNA according to  claim 2 .  
   
   
       9 . A vector according to  claim 8  wherein said DNA is operatively linked to a DNA expression control sequence.  
   
   
       10 . A host cell stably transformed or transfected with a DNA according to  claim 1  in a manner allowing expression of said DNA.  
   
   
       11 . A method for producing an 88-2B polypeptide comprising the steps of growing a host cell according to  claim 10  in a suitable nutrient medium and isolating said polypeptide from said cell or medium.  
   
   
       12 . A polynucleotide encoding an 88-2B polypeptide wherein said polynucleotide hybridizes under stringent hybridization conditions to the polynucleotide of SEQ ID NO: 3.  
   
   
       13 . A purified and isolated polypeptide comprising the chemokine receptor 88-2B amino acid sequence set out in SEQ ID NO:4.  
   
   
       14 . An antibody product that specifically binds a polypeptide comprising the 88-2B amino acid sequence set out in SEQ ID, NO:4.  
   
   
       15 . A hybridoma producing an antibody product according to  claim 14 .  
   
   
       16 . A purified and isolated polynucleotide encoding the amino acid sequence of chemokine receptor 88C set out in SEQ ID NO:2.  
   
   
       17 . A polynucleotide according to  claim 16  wherein the polynucleotide is DNA.  
   
   
       18 . A polynucleotide according to  claim 17  wherein the polynucleotide is genomic DNA.  
   
   
       19 . A polynucleotide according to  claim 17  wherein the polynucleotide is a cDNA.  
   
   
       20 . A polynucleotide according to  claim 16  which is a wholly or partially chemically synthesized DNA.  
   
   
       21 . An RNA transcript of the polynucleotide of  claim 17 .  
   
   
       22 . A cDNA according to  claim 19  comprising the DNA of SEQ ID NO:1.  
   
   
       23 . A biologically functional DNA vector comprising a DNA according to  claim 17 .  
   
   
       24 . A vector according to  claim 23  wherein said DNA is operatively linked to a DNA expression control sequence.  
   
   
       25 . A host cell stably transformed or transfected with a DNA according to  claim 16  in a manner allowing expression of said DNA.  
   
   
       26 . A method for producing an 88C polypeptide comprising the steps of growing a host cell according to  claim 25  in a suitable nutrient medium and isolating said polypeptide from said cell or medium.  
   
   
       27 . A polynucleotide encoding an 88C polypeptide wherein said polynucleotide hybridizes under stringent hybridization conditions to the polynucleotide of SEQ ID NO: 1.  
   
   
       28 . A purified and isolated polypeptide comprising the chemokine receptor 88C amino acid sequence set out in SEQ ID NO:2.  
   
   
       29 . An antibody product that specifically binds a polypeptide comprising the 88C amino acid sequence set out in SEQ ID NO:2.  
   
   
       30 . A hybridoma producing an antibody product according to  claim 29 .  
   
   
       31 . Hybridoma cell line 227K.  
   
   
       32 . Hybridoma cell line 227M.  
   
   
       33 . Hybridoma cell line 227N.  
   
   
       34 . Hybridoma cell line 227P.  
   
   
       35 . Hybridoma cell line 227R.  
   
   
       36 . A purified and isolated polynucleotide encoding the amino acid sequence of macqaque chemokine receptor 88C set out in SEQ ID NO: 20.  
   
   
       37 . A polynucleotide according to  claim 36  wherein the polynucleotide is DNA.  
   
   
       38 . A polynucleotide according to  claim 37  wherein the polynucleotide is genomic DNA.  
   
   
       39 . A polynucleotide according to  claim 37  wherein the polynucleotide is a cDNA.  
   
   
       40 . A polynucleotide according to  claim 36  which is a wholly or partially chemically synthesized DNA.  
   
   
       41 . An RNA transcript of the polynucleotide of  claim 37 .  
   
   
       42 . A cDNA according to  claim 39  comprising the DNA of SEQ ID NO:1.  
   
   
       43 . A biologically functional DNA vector comprising a DNA according to  claim 37 .  
   
   
       44 . A vector according to  claim 43  wherein said DNA is operatively linked to a DNA expression control sequence.  
   
   
       45 . A host cell stably transformed or transfected with a DNA according to  claim 36  in a manner allowing expression of said DNA.  
   
   
       46 . A method for producing a macqaque 88C polypeptide comprising the steps of growing a host cell according to  claim 45  in a suitable nutrient medium and isolating said polypeptide from said cell or medium.  
   
   
       47 . A polynucleotide encoding an 88C polypeptide wherein said polynucleotide hybridizes under stringent hybridization conditions to the polynucleotide of SEQ ID NO: 19.  
   
   
       48 . A purified and isolated polypeptide comprising the macqaque chemokine receptor 88C amino acid sequence set out in SEQ ID NO:20.  
   
   
       49 . An antibody product that specifically binds a polypeptide comprising the 88C amino acid sequence set out in SEQ ID NO:20.  
   
   
       50 . A hybridoma producing an antibody product according to  claim 49.

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